Dataset Information

Dataset of Cavendish banana transcriptome in response to chitosan coating application.

ABSTRACT: Banana is a climacteric fruit and its ripening process is greatly influenced by presence of ethylene. This physiological climacteric characteristic of banana fruit leads to a fast ripening and a short shelf-life. Application of edible coating such as chitosan aims to prolong fruit shelf life. The knowledge on gene expression will help to understand the fruit ripening process itself and chitosan effect on global gene expression. Global gene expression data of chitosan treated and control of Cavendish banana during fruit ripening were provided. Total RNA was isolated from banana pulp for differential gene expression analysis. The RNA-sequencing generated ranged from 16,155,947 to 23,587,110 total reads, with 75.8%-83.8% of reads were mapped against the genome reference. In total, 33,797-35,944 transcripts were detected. The transcriptomics data discussed in this publication are accessible through NCBI's Gene Expression Omnibus with GEO Series accession number GSE139457. These data provide information to identify candidate genes involved in fruit ripening in response to chitosan coating to design a better banana postharvest management.

SUBMITTER: Dwivany FM

PROVIDER: S-EPMC7066065 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Dataset of Cavendish banana transcriptome in response to chitosan coating application.

Dwivany Fenny Martha FM Nugrahapraja Husna H Fukusaki Eiichiro E Putri Sastia Prama SP Novianti Cindy C Radjasa Septhy Kusuma SK Fauziah Tessa T Nirmala Sari Lutfi Dewi LD

Data in brief 20200226

Banana is a climacteric fruit and its ripening process is greatly influenced by presence of ethylene. This physiological climacteric characteristic of banana fruit leads to a fast ripening and a short shelf-life. Application of edible coating such as chitosan aims to prolong fruit shelf life. The knowledge on gene expression will help to understand the fruit ripening process itself and chitosan effect on global gene expression. Global gene expression data of chitosan treated and control of Caven ...[more]

PMID: 32181300

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Project description:BACKGROUND: Fusarium wilt, caused by the fungal pathogen Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), is considered the most lethal disease of Cavendish bananas in the world. The disease can be managed in the field by planting resistant Cavendish plants generated by somaclonal variation. However, little information is available on the genetic basis of plant resistance to Foc TR4. To a better understand the defense response of resistant banana plants to the Fusarium wilt pathogen, the transcriptome profiles in roots of resistant and susceptible Cavendish banana challenged with Foc TR4 were compared. RESULTS: RNA-seq analysis generated more than 103 million 90-bp clean pair end (PE) reads, which were assembled into 88,161 unigenes (mean size = 554 bp). Based on sequence similarity searches, 61,706 (69.99%) genes were identified, among which 21,273 and 50,410 unigenes were assigned to gene ontology (GO) categories and clusters of orthologous groups (COG), respectively. Searches in the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG) mapped 33,243 (37.71%) unigenes to 119 KEGG pathways. A total of 5,008 genes were assigned to plant-pathogen interactions, including disease defense and signal transduction. Digital gene expression (DGE) analysis revealed large differences in the transcriptome profiles of the Foc TR4-resistant somaclonal variant and its susceptible wild-type. Expression patterns of genes involved in pathogen-associated molecular pattern (PAMP) recognition, activation of effector-triggered immunity (ETI), ion influx, and biosynthesis of hormones as well as pathogenesis-related (PR) genes, transcription factors, signaling/regulatory genes, cell wall modification genes and genes with other functions were analyzed and compared. The results indicated that basal defense mechanisms are involved in the recognition of PAMPs, and that high levels of defense-related transcripts may contribute to Foc TR4 resistance in banana. CONCLUSIONS: This study generated a substantial amount of banana transcript sequences and compared the defense responses against Foc TR4 between resistant and susceptible Cavendish bananas. The results contribute to the identification of candidate genes related to plant resistance in a non-model organism, banana, and help to improve the current understanding of host-pathogen interactions.

Dataset Information

Dataset of Cavendish banana transcriptome in response to chitosan coating application.

Publications

Dataset of Cavendish banana transcriptome in response to chitosan coating application.

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