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CSDE1 controls gene expression through the miRNA-mediated decay machinery.

ABSTRACT: In animals, miRNAs are the most prevalent small non-coding RNA molecules controlling posttranscriptional gene regulation. The Argonaute proteins (AGO) mediate miRNA-guided gene silencing by recruiting multiple factors involved in translational repression, deadenylation, and decapping. Here, we report that CSDE1, an RNA-binding protein linked to stem cell maintenance and metastasis in cancer, interacts with AGO2 within miRNA-induced silencing complex and mediates gene silencing through its N-terminal domains. We show that CSDE1 interacts with LSM14A, a constituent of P-body assembly and further associates to the DCP1-DCP2 decapping complex, suggesting that CSDE1 could promote the decay of miRNA-induced silencing complex-targeted mRNAs. Together, our findings uncover a hitherto unknown mechanism used by CSDE1 in the control of gene expression mediated by the miRNA pathway.

SUBMITTER: Kakumani PK

PROVIDER: S-EPMC7067469 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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CSDE1 controls gene expression through the miRNA-mediated decay machinery.

Kakumani Pavan Kumar PK Harvey Louis-Mathieu LM Houle François F Guitart Tanit T Gebauer Fátima F Simard Martin J MJ

Life science alliance 20200311 4

In animals, miRNAs are the most prevalent small non-coding RNA molecules controlling posttranscriptional gene regulation. The Argonaute proteins (AGO) mediate miRNA-guided gene silencing by recruiting multiple factors involved in translational repression, deadenylation, and decapping. Here, we report that CSDE1, an RNA-binding protein linked to stem cell maintenance and metastasis in cancer, interacts with AGO2 within miRNA-induced silencing complex and mediates gene silencing through its N-term ...[more]

PMID: 32161113

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