Electrodeposited Assembly of Additive-Free Silk Fibroin Coating from Pre-Assembled Nanospheres for Drug Delivery.

ABSTRACT: Electrophoretically deposited (EPD) polymer-based coatings have been extensively reported as reservoirs in medical devices for delivery of therapeutic agents, but control over drug release remains a challenge. Here, a simple but uncommon assembly strategy for EPD polymer coatings was proposed to improve drug release without introducing any additives except the EPD matrix polymer precursor. The added value of the proposed strategy was demonstrated by developing a novel EPD silk fibroin (SF) coating assembled from pre-assembled SF nanospheres for an application model, that is, preventing infections around percutaneous orthopedic implants via local delivery of antibiotics. The EPD mechanism of this nanosphere coating involved oxidation of water near the substrate to neutralize SF nanospheres, resulting in irreversible deposition. The deposition process and mass could be easily controlled using the applied EPD parameters. In comparison with the EPD SF coating assembled in a conventional way (directly obtained from SF molecule solutions), this novel coating had a similar adhesion strength but exhibited a more hydrophobic nanotopography to induce better fibroblastic response. Moreover, the use of nanospheres as building blocks enabled 1.38 and 21 times enhancement on the antibiotic release amount and time (of 95% maximum dug release), respectively, while retaining drug effectiveness and showing undetectable cytotoxicity. This unexpected release kinetics was found attributable to the electrostatic and hydrophobic interactions between the drug and nanospheres and a negligible initial dissolution effect on the nanosphere coating. These results illustrate the promising potential of the pre-assembled strategy on EPD polymer coatings for superior control over drug delivery.

SUBMITTER: Cheng X 

PROVIDER: S-EPMC7068717 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature