N6-methyladenosine-induced ERR? triggers chemoresistance of cancer cells through upregulation of ABCB1 and metabolic reprogramming.

ABSTRACT: Background: Drug resistance severely reduces treatment efficiency of chemotherapy and leads to poor prognosis. However, regulatory factors of chemoresistant cancer cells are largely unknown. Methods: The expression of estrogen receptor related receptors (ERRs) in chemoresistant cancer cells are checked. The roles of ERR? in chemoresistance are confirmed by in vitro and in vivo studies. The mechanisms responsible for ERR?-regulated expression of ABCB1 and CPT1B are investigated. Results: The expression of ERR? is upregulated in chemoresistant cancer cells. Targeted inhibition of ERR? restores the chemosensitivity. ERR? can directly bind to the promoter of ABCB1 to increase its transcription. An elevated interaction between ERR? and p65 in chemoresistant cells further strengthens transcription of ABCB1. Further, ERR? can increase the fatty acid oxidation (FAO) in chemoresistant cells via regulation of CPT1B, the rate-limiting enzyme of FAO. The upregulated ERR? in chemoresistant cancer cells might be due to increased levels of N6-methyladenosine (m6A) can trigger the splicing of precursor ESRRG mRNA. Conclusions: m6A induced ERR? confers chemoresistance of cancer cells through upregulation of ABCB1 and CPT1B.

SUBMITTER: Chen Z 

PROVIDER: S-EPMC7069076 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature