Ontology highlight
ABSTRACT:
SUBMITTER: Flynn JM
PROVIDER: S-EPMC7069724 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Flynn Julia M JM Rossouw Ammeret A Cote-Hammarlof Pamela P Fragata Inês I Mavor David D Hollins Carl C Bank Claudia C Bolon Daniel Na DN
eLife 20200304
Gene-environment interactions have long been theorized to influence molecular evolution. However, the environmental dependence of most mutations remains unknown. Using deep mutational scanning, we engineered yeast with all 44,604 single codon changes encoding 14,160 amino acid variants in Hsp90 and quantified growth effects under standard conditions and under five stress conditions. To our knowledge, these are the largest determined comprehensive fitness maps of point mutants. The growth of many ...[more]