Dataset Information

Determination of pyrethroid residues in herbal tea using temperature-controlled ionic liquid dispersive liquid-liquid microextraction by high performance liquid chromatography.

ABSTRACT: A simple and effective method for determining five pyrethroid residues in herbal tea by ultrasound-enhanced temperature-controlled (UETC) ionic liquid dispersive liquid-liquid microextraction (IL-DLLME) coupled with high performance liquid chromatography-diode array detection (HPLC-DAD) was developed. The use of ultrasonication and heating improved the ability of the ionic liquid to extract the analytes. Various parameters that affect the extraction efficiency were investigated and optimized using single factor experiments and response surface design. The optimum conditions of the experiment were 121?µL of [HMIM][PF6] (extraction solvent), 794?µL of acetonitrile (dispersive solvent), a heating temperature of 40°C, a sonication time of 3.6?min and a pH of 2.9. Under optimized conditions, the linearity was in the range of 0.05-5?mg?L-1 with correlation coefficients above 0.9993. The limits of detection and quantification were 1.25-1.35?µg?L-1 and 5?µg?L-1, respectively. The mean recoveries of the five pyrethroids ranged from 74.02% to 109.01%, with RSDs below 9.04%. The proposed method was reliable for the analysis of pyrethroids in Chinese herbal tea.

SUBMITTER: Zhang R

PROVIDER: S-EPMC7070011 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Determination of pyrethroid residues in herbal tea using temperature-controlled ionic liquid dispersive liquid-liquid microextraction by high performance liquid chromatography.

Zhang Rui R Tan Zhenchao Z Zhao Junlong J Wen Yan Y Fan Shuai S Liu Chenglan C

Scientific reports 20200313 1

A simple and effective method for determining five pyrethroid residues in herbal tea by ultrasound-enhanced temperature-controlled (UETC) ionic liquid dispersive liquid-liquid microextraction (IL-DLLME) coupled with high performance liquid chromatography-diode array detection (HPLC-DAD) was developed. The use of ultrasonication and heating improved the ability of the ionic liquid to extract the analytes. Various parameters that affect the extraction efficiency were investigated and optimized usi ...[more]

PMID: 32170197

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Project description:An ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME) of 20 anthelmintic drugs followed and detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed, optimized, and validated. The parameters affecting the anthelmintic extraction efficiencies such as selection of extraction solvent (ionic liquids), selection of disperser solvent, volume of extraction solvent, volume of disperser solvent, pH of the aqueous phase, extraction time, salt addition, and centrifugation time were optimized. Validation was conducted according to ISO/IEC 17025:2017 and Commission Implementing Regulation (EU) 2021/808 of 22 March 2021. Validation parameters such as calibration function, matrix effect, limit of detection (LOD), limit of quantification (LOQ), decision limit (CCα), accuracy, and precision were established. Coefficient of determination (R 2) values ranging from .99938 to .99995 were obtained using the matrix calibration curve spiked at 0, 0.25, 1.0, 1.5, and 2.0 times MRL. The LODs and LOQs were calculated using the standard deviation of the response and the slopes of the calibration curves ranged from 0.35 to 26.1 μg/kg and from 1.2 to 87.0 μg/kg, respectively, and were dependent on calibration range. The CCα values ranged from 23 to 1022.0 μg/kg and are also dependent on the MRL concentration levels. The coefficient of variation (CV) values calculated are within the reproducibility range of 16%-30% adapted from the Horwitz Equation CV = 2(1-0.5 log C) and ranged from 1.7% to 16.9%. The developed and validated and the standard QuEChERS method were compared. The IL-DLLME LC-MS/MS method was applied to 32 small stock (18 caprine [goat] and 14 ovine [sheep]) liver samples received from municipal abattoirs at Botswana National Veterinary Laboratory for the analysis of anthelmintic drug residues. The results obtained indicated that the anthelmintic drug residues were all below the detection capability, and therefore, the samples were passed as fit for human consumption.

Dataset Information

Determination of pyrethroid residues in herbal tea using temperature-controlled ionic liquid dispersive liquid-liquid microextraction by high performance liquid chromatography.

Publications

Determination of pyrethroid residues in herbal tea using temperature-controlled ionic liquid dispersive liquid-liquid microextraction by high performance liquid chromatography.

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