Paracetamol is a centrally acting analgesic using mechanisms located in the periaqueductal grey.

ABSTRACT:

Background and purpose

We previously demonstrated that paracetamol has to be metabolised in the brain by fatty acid amide hydrolase enzyme into AM404 (N-(4-hydroxyphenyl)-5Z,8Z,11Z,14Z-eicosatetraenamide) to activate CB₁ receptors and TRPV1 channels, which mediate its analgesic effect. However, the brain mechanisms supporting paracetamol-induced analgesia remain unknown.

Experimental approach

The effects of paracetamol on brain function in Sprague-Dawley rats were determined by functional MRI. Levels of neurotransmitters in the periaqueductal grey (PAG) were measured using in vivo ¹ H-NMR and microdialysis. Analgesic effects of paracetamol were assessed by behavioural tests and challenged with different inhibitors, administered systemically or microinje

SUBMITTER: Barriere DA 

PROVIDER: S-EPMC7070177 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature