Ontology highlight
ABSTRACT:
SUBMITTER: Ruetz M
PROVIDER: S-EPMC7070230 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Ruetz Markus M Campanello Gregory C GC Purchal Meredith M Shen Hongying H McDevitt Liam L Gouda Harsha H Wakabayashi Shoko S Zhu Junhao J Rubin Eric J EJ Warncke Kurt K Mootha Vamsi K VK Koutmos Markos M Banerjee Ruma R
Science (New York, N.Y.) 20191101 6465
Itaconate is an immunometabolite with both anti-inflammatory and bactericidal effects. Its coenzyme A (CoA) derivative, itaconyl-CoA, inhibits B<sub>12</sub>-dependent methylmalonyl-CoA mutase (MCM) by an unknown mechanism. We demonstrate that itaconyl-CoA is a suicide inactivator of human and <i>Mycobacterium tuberculosis</i> MCM, which forms a markedly air-stable biradical adduct with the 5'-deoxyadenosyl moiety of the B<sub>12</sub> coenzyme. Termination of the catalytic cycle in this way imp ...[more]