Unknown

Dataset Information

0

Gene Expression Profiling in Ovaries and Association Analyses Reveal HEP21 as a Candidate Gene for Sexual Maturity in Chickens.

ABSTRACT: The age of onset of sexual maturity is an important reproductive trait in chickens. In this study, we explored candidate genes associated with sexual maturity and ovary development in chickens. We performed DGE RNA-sequencing analyses of ovaries of pre-laying (P-F-O1, L-F-O1) and laying (P-F-O2, L-F-O2) hens of two sub-breeds of Ningdu Yellow chicken. A total of 3197 genes were identified in the two comparisons, and 966 and 1860 genes were detected exclusively in comparisons of P-F-O1 vs. P-F-O2 and L-F-O1 vs. L-F-O2, respectively. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that genes involved in transmembrane signaling receptor activity, cell adhesion, developmental processes, the neuroactive ligand-receptor interaction pathway, and the calcium signaling pathway were enriched in both comparisons. Genes on these pathways, including growth hormone (GH), integrin subunit beta 3 (ITGB3), thyroid stimulating hormone subunit beta (TSHB), prolactin (PRL), and transforming growth factor beta 3 (TGFB3), play indispensable roles in sexual maturity. As a gene unique to poultry, hen egg protein 21 kDa (HEP21) was chosen as the candidate gene. Differential expression and association analyses were performed. RNA-seq data and qPCR showed that HEP21 was significantly differentially expressed in pre-pubertal and pubertal ovaries. A total of 23 variations were detected in HEP21. Association analyses of single nucleotide polymorphisms (SNPs) in HEP21 and reproductive traits showed that rs315156783 was significantly related to comb height at 84 and 91 days. These results indicate that HEP21 is a candidate gene for sexual maturity in chickens. Our results contribute to a more comprehensive understanding of sexual maturity and reproduction in chickens.

SUBMITTER: Chen B 

PROVIDER: S-EPMC7071030 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Gene Expression Profiling in Ovaries and Association Analyses Reveal <i>HEP21</i> as a Candidate Gene for Sexual Maturity in Chickens.

Chen Biao B   Liang Guitao G   Zhu Xuenong X   Tan Yuwen Y   Xu Jiguo J   Wu Hongxiang H   Mao Huirong H   Zhang Yutao Y   Chen Jiakun J   Rao Yousheng Y   Zhou Min M   Liu Sanfeng S  

Animals : an open access journal from MDPI 20200121 2

The age of onset of sexual maturity is an important reproductive trait in chickens. In this study, we explored candidate genes associated with sexual maturity and ovary development in chickens. We performed DGE RNA-sequencing analyses of ovaries of pre-laying (P-F-O1, L-F-O1) and laying (P-F-O2, L-F-O2) hens of two sub-breeds of Ningdu Yellow chicken. A total of 3197 genes were identified in the two comparisons, and 966 and 1860 genes were detected exclusively in comparisons of P-F-O1 vs. P-F-O2  ...[more]

Similar Datasets

Transcriptomics Gene Expression Profiling in ovaries and association analysis reveal HEP21 as a candidate gene for chicken sexual maturity
2020-03-23 | GSE136329 | GEO
Genomics Gene Expression Profiling in ovaries and association analysis reveal HEP21 as a candidate gene for chicken sexual maturity
| PRJNA563042 | ENA
Unknown Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity.
| S-EPMC6434470 | biostudies-literature
Unknown RNA Sequencing of the Pituitary Gland and Association Analyses Reveal <i>PRKG2</i> as a Candidate Gene for Growth and Carcass Traits in Chinese Ningdu Yellow Chickens.
| S-EPMC9244401 | biostudies-literature
Unknown Genome-Wide Association and RNA-Seq Analyses Reveal a Potential Candidate Gene Related to Oil Content in Soybean Seeds.
| S-EPMC11311756 | biostudies-literature
Unknown Genome-wide association study and gene network analyses reveal potential candidate genes for high night temperature tolerance in rice.
| S-EPMC7991035 | biostudies-literature
Unknown Genomic analyses reveal the genetic basis of early maturity and identification of loci and candidate genes in upland cotton (Gossypium hirsutum L.).
| S-EPMC7769233 | biostudies-literature
Unknown Genetic and gene expression analyses of the polycystic ovary syndrome candidate gene fibrillin-3 and other fibrillin family members in human ovaries.
| S-EPMC2776474 | biostudies-literature
Unknown Genomic Analyses Reveal Genetic Adaptations to Tropical Climates in Chickens.
| S-EPMC7578744 | biostudies-literature
Unknown Genome analyses reveal population structure and a purple stigma color gene candidate in finger millet.
| S-EPMC10284860 | biostudies-literature