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Cymbopogon Citratus Functionalized Green Synthesis of CuO-Nanoparticles: Novel Prospects as Antibacterial and Antibiofilm Agents.

ABSTRACT: Chemically synthesized copper oxide nanoparticles (CuONPs) involve the generation of toxic products, which narrowed its biological application. Hence, we have developed a one-pot, green method for CuONP production employing the leaf extract of Cymbopogon citratus (CLE). Gas chromatography-mass spectrometry (GC-MS) analysis confirmed the capping of CuONPs by CLE esters (CLE-CuONPs). Fourier-transform infrared (FTIR) showed phenolics, sugars, and proteins mediated nucleation and stability of CLE-CuONPs. X-ray diffraction (XRD) and transmission electron microscopy (TEM) revealed CLE-CuONPs between 11.4 to 14.5 nm. Staphylococcus aureus-1 (MRSA-1), Staphylococcus aureus-2 (MSSA-2) exposed to CLE-CuONPs (1500 µg/mL) showed 51.4%, 32.41% survival, while Escherichia coli-336 (E. coli-336) exposed to 1000 µg/mL CLE-CuONPs showed 45.27% survival. Scanning electron microscopy (SEM) of CLE-CuONPs treated E. coli-336, MSSA-2 and MRSA-1 showed morphological deformations. The biofilm production by E. coli-336 and MRSA-1 also declined to 33.0 ± 3.2% and 49.0 ± 3.1% at 2000 µg/mL of CLE-CuONPs. Atomic absorption spectroscopy (AAS) showed 22.80 ± 2.6%, 19.2 ± 4.2%, and 16.2 ± 3.6% accumulation of Cu2+ in E. coli-336, MSSA-2, and MRSA-1. Overall, the data exhibited excellent antibacterial and antibiofilm efficacies of esters functionalized CLE-CuONPs, indicating its putative application as a novel nano-antibiotic against multi drug resistance (MDR) pathogenic clinical isolates.

SUBMITTER: Cherian T

PROVIDER: S-EPMC7072505 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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<i>Cymbopogon Citratus</i> Functionalized Green Synthesis of CuO-Nanoparticles: Novel Prospects as Antibacterial and Antibiofilm Agents.

Cherian Tijo T Ali Khursheed K Saquib Quaiser Q Faisal Mohammad M Wahab Rizwan R Musarrat Javed J

Biomolecules 20200122 2

Chemically synthesized copper oxide nanoparticles (CuONPs) involve the generation of toxic products, which narrowed its biological application. Hence, we have developed a one-pot, green method for CuONP production employing the leaf extract of <i>Cymbopogon citratus</i> (CLE). Gas chromatography-mass spectrometry (GC-MS) analysis confirmed the capping of CuONPs by CLE esters (CLE-CuONPs). Fourier-transform infrared (FTIR) showed phenolics, sugars, and proteins mediated nucleation and stability o ...[more]

PMID: 31979040

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Project description:Excessive use of antimicrobial medications including antibiotics has led to the emerging menace of antimicrobial resistance, which, as per the World Health Organization (WHO), is among the top ten public health threats facing humanity, globally. This necessitates that innovative technologies be sought that can aid in the elimination of pathogens and hamper the spread of infections. Zinc oxide (ZnO) has multifunctionality owing to its extraordinary physico-chemical properties and functionality in a range of applications. In this research, ZnO nanoparticles (NPs) were synthesized from zinc nitrate hexahydrate, by a green synthesis approach using Cymbopogon citratus extract followed by characterization of the NPs. The obtained X-ray diffraction peaks of ZnO NPs matched with the standard JCPDS card (no. 89-510). The particles had a size of 20-24 nm, a wurtzite structure with a high crystallinity, and hexagonal rod-like shape. UV-Vis spectroscopy revealed absorption peaks between 369 and 374 nm of ZnO NPs synthesized from C. citratus extract confirming the formation of ZnO. Fourier transform infrared confirmed the ZnO NPs as strong absorption bands were observed in the range of 381-403 cm-1 corresponding to Zn-O bond stretching. Negative values of the highest occupied molecular orbital-lowest unoccupied molecular orbital for ZnO NPs indicated the good potential to form a stable ligand-protein complex. Docking results indicated favorable binding interaction between ZnO and DNA gyrase subunit b with a binding energy of -2.93 kcal/mol. ZnO NPs at various concentrations inhibited the growth of Escherichia coli and Staphylococcus aureus. Minimum inhibitory concentration values of ZnO NPs against E. coli and S. aureus were found to be 92.07 ± 0.13 and 88.13 ± 0.35 μg/mL, respectively, at a concentration of 2 mg/mL. AO/EB staining and fluorescence microscopy revealed the ability of ZnO NPs to kill E. coli and S. aureus cells. Through the findings of this study, it has been shown that C. citratus extract can be used in a green synthesis approach to generate ZnO NPs, which can be employed as alternatives to antibiotics and a tool to eliminate drug-resistant microbes in the future.

Project description:Cymbopogon citratus (DC) stapf. (Gramineae) is a herb known worldwide as lemongrass. The oil obtained, i.e., lemongrass oil has emerged as one among the most relevant natural oils in the pharmaceutical industry owing to its extensive pharmacological and therapeutic benefits including antioxidant, antimicrobial, antiviral and anticancer properties. However, its usage in novel formulations is constrained because of its instability and volatility. To address these concerns, the present study aims to formulate lemongrass-loaded SLN (LGSLN) using hot water titration technique. In the Smix, Tween 80 was selected as a surfactant component, while ethanol was taken as a co-surfactant. Different ratios of Smix (1:1, 1:2, 1:3, 2:1 and 3:1) were utilized to formulate LG-loaded SLN. The results indicated the fact that the LGSLN formulation (abbreviated as LGSLN1), containing lipid phase 10% w/w (i.e., LG 3.33% and SA 6.67%), Tween 80 (20% w/w), ethanol (20% w/w) and distilled water (50% w/w), revealed suitable nanometric size (142.3 ± 5.96 nm) with a high zeta potential value (- 29.12 ± 1.7 mV) and a high entrapment efficiency (77.02 ± 8.12%). A rapid drug release (71.65 ± 5.33%) was observed for LGSLN1 in a time span of 24 h. Additionally, the highest values for steady-state flux (Jss; 0.6133 ± 0.0361 mg/cm2/h), permeability coefficient (Kp; 0.4573 ± 0.0141 (cm/h) × 102) and enhancement ratio (Er; 13.50) was also conferred by LGSLN1. Based on in vitro study results, the developed SLN appeared as a potential carrier for enhanced topical administration of lemongrass oil. The observed results also indicated the fact that the phyto-cosmeceutical prospective of the nanolipidic carrier for topical administration of lemongrass oil utilizing pharmaceutically acceptable components can be explored further for widespread clinical applicability.Supplementary informationThe online version contains supplementary material available at 10.1007/s13205-023-03726-5.

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Cymbopogon Citratus Functionalized Green Synthesis of CuO-Nanoparticles: Novel Prospects as Antibacterial and Antibiofilm Agents.

Publications

<i>Cymbopogon Citratus</i> Functionalized Green Synthesis of CuO-Nanoparticles: Novel Prospects as Antibacterial and Antibiofilm Agents.

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