Unknown

Dataset Information

0

Dual-procedural separation of CTCs in cutaneous melanoma provides useful information for both molecular diagnosis and prognosis.


ABSTRACT: Background:Circulating tumor cells (CTCs) have recently emerged as a new dynamic soluble marker for several malignancies including cutaneous melanoma (CM) and are suitable for prognostic evaluations and treatment monitoring. However, to date many limitations still hamper the wide-scale application of CTCs in CM setting, including the lack of standardized methods as well as both low levels and heterogeneity of these cells. Methods:We developed a protocol for CTC detection in CM based on immune-magnetic sorting to deplete CD45-, CD31- or CD34-positive cells, followed by dielectrophoretic DEPArray separation according to cell morphology and immunophenotype. To this end, we explored the expression of melanoma stem cell antigens (CD271, ABCB5, and RANK) and the epithelial-to-mesenchymal transition markers (N-Cad, -CD44, and -MCAM/CD146) on CTCs from 17 stage IV CM patients, and investigated their BRAF mutational status by droplet digital PCR. Results:The number of CTCs isolated from CM patients ranged from 2 to 91 cells (38?±?6.4) with respect to healthy donors (p?

SUBMITTER: Tucci M 

PROVIDER: S-EPMC7074504 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

Dual-procedural separation of CTCs in cutaneous melanoma provides useful information for both molecular diagnosis and prognosis.

Tucci Marco M   D'Oronzo Stella S   Mannavola Francesco F   Felici Claudia C   Lovero Domenica D   Cafforio Paola P   Palmirotta Raffaele R   Silvestris Franco F  

Therapeutic advances in medical oncology 20200313


<h4>Background</h4>Circulating tumor cells (CTCs) have recently emerged as a new dynamic soluble marker for several malignancies including cutaneous melanoma (CM) and are suitable for prognostic evaluations and treatment monitoring. However, to date many limitations still hamper the wide-scale application of CTCs in CM setting, including the lack of standardized methods as well as both low levels and heterogeneity of these cells.<h4>Methods</h4>We developed a protocol for CTC detection in CM bas  ...[more]

Similar Datasets

| S-EPMC3180927 | biostudies-literature
| S-EPMC5694001 | biostudies-literature
| S-EPMC10497583 | biostudies-literature
| S-EPMC10150491 | biostudies-literature
| S-EPMC9525058 | biostudies-literature
2024-02-14 | GSE238207 | GEO
| S-EPMC7289430 | biostudies-literature
| S-EPMC7718036 | biostudies-literature
| S-EPMC10213036 | biostudies-literature
| S-EPMC4306428 | biostudies-literature