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Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets.


ABSTRACT: : Here, we present a miniaturized lab-on-a-chip detecting system for an all-electric and label-free analysis of the emulsion droplets incorporating the nanoscopic silicon nanowires-based field-effect transistors (FETs). We specifically focus on the analysis of ?-galactosidase e.g.activity, which is an important enzyme of the glycolysis metabolic pathway. Furthermore, the efficiency of the synthesis and action of ?-galactosidase can be one of the markers for several diseases, e.g., cancer, hyper/hypoglycemia, cell senescence, or other disruptions in cell functioning. We measure the reaction and reaction kinetics-associated shift of the source-to-drain current Isd in the system, which is caused by the change of the ionic strength of the microenvironment. With these results, we demonstrate that the ion-sensitive FETs are able to sense the interior of the aqueous reactors; thus, the conjunction of miniature nanosensors and droplet-based microfluidic systems conceptually opens a new route toward a sensitive, optics-less analysis of biochemical processes.

SUBMITTER: Belyaev D 

PROVIDER: S-EPMC7074628 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets.

Belyaev Dmitry D   Schütt Julian J   Ibarlucea Bergoi B   Rim Taiuk T   Baraban Larysa L   Cuniberti Gianaurelio G  

Micromachines 20200126 2


<b>:</b> Here, we present a miniaturized lab-on-a-chip detecting system for an all-electric and label-free analysis of the emulsion droplets incorporating the nanoscopic silicon nanowires-based field-effect transistors (FETs). We specifically focus on the analysis of β-galactosidase <i>e.g.</i>activity, which is an important enzyme of the glycolysis metabolic pathway. Furthermore, the efficiency of the synthesis and action of β-galactosidase can be one of the markers for several diseases, <i>e.g  ...[more]

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