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Organic electrochemical transistor arrays for real-time mapping of evoked neurotransmitter release in vivo.


ABSTRACT: Though neurotransmitters are essential elements in neuronal signal transduction, techniques for in vivo analysis are still limited. Here, we describe an organic electrochemical transistor array (OECT-array) technique for monitoring catecholamine neurotransmitters (CA-NTs) in rat brains. The OECT-array is an active sensor with intrinsic amplification capability, allowing real-time and direct readout of transient CA-NT release with a sensitivity of nanomolar range and a temporal resolution of several milliseconds. The device has a working voltage lower than half of that typically used in a prevalent cyclic voltammetry measurement, and operates continuously in vivo for hours without significant signal drift, which is inaccessible for existing methods. With the OECT-array, we demonstrate simultaneous mapping of evoked dopamine release at multiple striatal brain regions in different physiological scenarios, and reveal a complex cross-talk between the mesolimbic and the nigrostriatal pathways, which is heterogeneously affected by the reciprocal innervation between ventral tegmental area and substantia nigra pars compacta.

SUBMITTER: Xie K 

PROVIDER: S-EPMC7075691 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Organic electrochemical transistor arrays for real-time mapping of evoked neurotransmitter release in vivo.

Xie Kai K   Wang Naixiang N   Lin Xudong X   Wang Zixun Z   Zhao Xi X   Fang Peilin P   Yue Haibing H   Kim Junhwi J   Luo Jing J   Cui Shaoyang S   Yan Feng F   Shi Peng P  

eLife 20200211


Though neurotransmitters are essential elements in neuronal signal transduction, techniques for <i>in vivo</i> analysis are still limited. Here, we describe an organic electrochemical transistor array (OECT-array) technique for monitoring catecholamine neurotransmitters (CA-NTs) in rat brains. The OECT-array is an <i>active</i> sensor with intrinsic amplification capability, allowing real-time and direct readout of transient CA-NT release with a sensitivity of nanomolar range and a temporal reso  ...[more]

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