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Gut microbiome diversity detected by high-coverage 16S and shotgun sequencing of paired stool and colon sample.

ABSTRACT: The gut microbiome has a fundamental role in human health and disease. However, studying the complex structure and function of the gut microbiome using next generation sequencing is challenging and prone to reproducibility problems. Here, we obtained cross-sectional colon biopsies and faecal samples from nine participants in our COLSCREEN study and sequenced them in high coverage using Illumina pair-end shotgun (for faecal samples) and IonTorrent 16S (for paired feces and colon biopsies) technologies. The metagenomes consisted of between 47 and 92 million reads per sample and the targeted sequencing covered more than 300 k reads per sample across seven hypervariable regions of the 16S gene. Our data is freely available and coupled with code for the presented metagenomic analysis using up-to-date bioinformatics algorithms. These results will add up to the informed insights into designing comprehensive microbiome analysis and also provide data for further testing for unambiguous gut microbiome analysis.

SUBMITTER: Mas-Lloret J

PROVIDER: S-EPMC7075950 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Gut microbiome diversity detected by high-coverage 16S and shotgun sequencing of paired stool and colon sample.

Mas-Lloret Joan J Obón-Santacana Mireia M Ibáñez-Sanz Gemma G Guinó Elisabet E Pato Miguel L ML Rodriguez-Moranta Francisco F Mata Alfredo A García-Rodríguez Ana A Moreno Victor V Pimenoff Ville Nikolai VN

Scientific data 20200316 1

The gut microbiome has a fundamental role in human health and disease. However, studying the complex structure and function of the gut microbiome using next generation sequencing is challenging and prone to reproducibility problems. Here, we obtained cross-sectional colon biopsies and faecal samples from nine participants in our COLSCREEN study and sequenced them in high coverage using Illumina pair-end shotgun (for faecal samples) and IonTorrent 16S (for paired feces and colon biopsies) technol ...[more]

PMID: 32179734

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Dataset Information

Gut microbiome diversity detected by high-coverage 16S and shotgun sequencing of paired stool and colon sample.

Publications

Gut microbiome diversity detected by high-coverage 16S and shotgun sequencing of paired stool and colon sample.

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