Unknown

Dataset Information

0

Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance.


ABSTRACT: Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) 'barcode' gene, which remains costly for purposes of screening large numbers of unknown samples during routine surveillance. Here, we adapted a rapid, low-cost approach to differentiate 10 domestic and 24 wildlife species that are common in the East African illegal wildlife products trade based on their unique high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR products. Using the approach, we identified (i) giraffe among covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences among savannah elephant reference samples. This approach is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines.

SUBMITTER: Ouso DO 

PROVIDER: S-EPMC7075967 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance.

Ouso Daniel O DO   Otiende Moses Y MY   Jeneby Maamun M MM   Oundo Joseph W JW   Bargul Joel L JL   Miller Scott E SE   Wambua Lillian L   Villinger Jandouwe J  

Scientific reports 20200316 1


Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) 'barcode' gene, which remains costly for purposes of screening large numbers of unknown sample  ...[more]

Similar Datasets

| S-EPMC7911181 | biostudies-literature
| S-EPMC7269394 | biostudies-literature
| S-EPMC8426997 | biostudies-literature
| S-EPMC5585169 | biostudies-literature
| S-EPMC10132831 | biostudies-literature
| S-EPMC9430602 | biostudies-literature
| S-EPMC7652797 | biostudies-literature
| S-EPMC8052663 | biostudies-literature
| S-EPMC2828872 | biostudies-literature
| S-EPMC4519940 | biostudies-literature