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Microbial insights of enhanced anaerobic conversion of syngas into volatile fatty acids by co-fermentation with carbohydrate-rich synthetic wastewater.

ABSTRACT: Background:The co-fermentation of syngas (mainly CO, H2 and CO2) and different concentrations of carbohydrate/protein synthetic wastewater to produce volatile fatty acids (VFAs) was conducted in the present study. Results:It was found that co-fermentation of syngas with carbohydrate-rich synthetic wastewater could enhance the conversion efficiency of syngas and the most efficient conversion of syngas was obtained by co-fermentation of syngas with 5 g/L glucose, which resulted in 25% and 43% increased conversion efficiencies of CO and H2, compared to syngas alone. The protein-rich synthetic wastewater as co-substrate, however, had inhibition on syngas conversion due to the presence of high concentration of NH4 +-N (> 900 mg/L) produced from protein degradation. qPCR analysis found higher concentration of acetogens, which could use CO and H2, was present in syngas and glucose co-fermentation system, compared to glucose solo-fermentation or syngas solo-fermentation. In addition, the known acetogen Clostridium formicoaceticum, which could utilize both carbohydrate and CO/H2 was enriched in syngas solo-fermentation and syngas with glucose co-fermentation. In addition, butyrate was detected in syngas and glucose co-fermentation system, compared to glucose solo-fermentation. The detected n-butyrate could be converted from acetate and lactate/ethanol which produced from glucose in syngas and glucose co-fermentation system supported by label-free quantitative proteomic analysis. Conclusions:These results demonstrated that the co-fermentation with syngas and carbohydrate-rich wastewater could be a promising technology to increase the conversion of syngas to VFAs. In addition, the syngas and glucose co-fermentation system could change the degradation pathway of glucose in co-fermentation and produce fatty acids with longer carbon chain supported by microbial community and label-free quantitative proteomic analysis. The above results are innovative and lead to achieve effective conversion of syngas into VFAs/longer chain fatty acids, which would for sure have a great interest for the scientific and engineering community. Furthermore, the present study also used the combination of high-throughput sequencing of 16S rRNA genes, qPCR analysis and label-free quantitative proteomic analysis to provide deep insights of the co-fermentation process from the taxonomic and proteomic aspects, which should be applied for future studies relating with anaerobic fermentation.

SUBMITTER: Liu C

PROVIDER: S-EPMC7076986 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Microbial insights of enhanced anaerobic conversion of syngas into volatile fatty acids by co-fermentation with carbohydrate-rich synthetic wastewater.

Liu Chao C Wang Wen W O-Thong Sompong S Yang Ziyi Z Zhang Shicheng S Liu Guangqing G Luo Gang G

Biotechnology for biofuels 20200316

<h4>Background</h4>The co-fermentation of syngas (mainly CO, H<sub>2</sub> and CO<sub>2</sub>) and different concentrations of carbohydrate/protein synthetic wastewater to produce volatile fatty acids (VFAs) was conducted in the present study.<h4>Results</h4>It was found that co-fermentation of syngas with carbohydrate-rich synthetic wastewater could enhance the conversion efficiency of syngas and the most efficient conversion of syngas was obtained by co-fermentation of syngas with 5 g/L glucos ...[more]

PMID: 32190118

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Dataset Information

Microbial insights of enhanced anaerobic conversion of syngas into volatile fatty acids by co-fermentation with carbohydrate-rich synthetic wastewater.

Publications

Microbial insights of enhanced anaerobic conversion of syngas into volatile fatty acids by co-fermentation with carbohydrate-rich synthetic wastewater.

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