On rare variants in principal component analysis of population stratification.

ABSTRACT:

Background

Population stratification is a known confounder of genome-wide association studies, as it can lead to false positive results. Principal component analysis (PCA) method is widely applied in the analysis of population structure with common variants. However, it is still unclear about the analysis performance when rare variants are used.

Results

We derive a mathematical expectation of the genetic relationship matrix. Variance and covariance elements of the expected matrix depend explicitly on allele frequencies of the genetic markers used in the PCA analysis. We show that inter-population variance is solely contained in K principal components (PCs) and mostly in the largest K-1 PCs, where K is the number of populations in the samples. We propose F_PC, ratio of the inter-population variance to the intra-population variance in the K population informative PCs, and d², sum of squared distances among populations, as measures of population divergence. We show analytically that when allele frequencies become small, the ratio F_PC abates, the population distance d² decreases, and portion of variance explained by the K PCs diminishes. The results are validated in the analysis of the 1000 Genomes Project data. The ratio F_PC is 93.85, population distance d² is 444.38, and variance explained by the largest five PCs is 17.09% when using with common variants with allele frequencies between 0.4 and 0.5. However, the ratio, distance and percentage decrease to 1.83, 17.83 and 0.74%, respectively, with rare variants of frequencies between 0.0001 and 0.01.

Conclusions

The PCA of population stratification performs worse with rare variants than with common ones. It is necessary to restrict the selection to only the common variants when analyzing population stratification with sequencing data.

SUBMITTER: Ma S 

PROVIDER: S-EPMC7077175 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature