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A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants.


ABSTRACT: A carboxylesterase (CXE) or carboxylic-ester hydrolase is an enzyme that catalyzes carboxylic ester and water into alcohol and carboxylate. In plants, CXEs have been implicated in defense, development, and secondary metabolism. We discovered a new CXE gene in Nicotiana benthamiana that is related to virus resistance. The transcriptional level of NbCXE expression was significantly increased after Tobacco mosaic virus (TMV) infection. Transient over-expression of NbCXE inhibited TMV accumulation in N. benthamiana plants. Conversely, when the NbCXE gene was silenced with a Tobacco rattle virus (TRV)-based gene silencing system, TMV RNA accumulation was increased in NbCXE-silenced plants after infection. NbCXE protein was shown to interact with TMV coat protein (CP) in vitro. Additionally, the expressions of host defense-related genes were increased in transient NbCXE-overexpressed plants but decreased in NbCXE silenced N. benthamiana plants. In summary, our study showed that NbCXE is a novel resistance-related gene involved in host defense responses against TMV infection.

SUBMITTER: Guo S 

PROVIDER: S-EPMC7077250 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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A Conserved Carboxylesterase Inhibits <i>Tobacco mosaic virus</i> (TMV) Accumulation in <i>Nicotiana benthamiana</i> Plants.

Guo Song S   Wong Sek-Man SM  

Viruses 20200210 2


A carboxylesterase (CXE) or carboxylic-ester hydrolase is an enzyme that catalyzes carboxylic ester and water into alcohol and carboxylate. In plants, CXEs have been implicated in defense, development, and secondary metabolism. We discovered a new CXE gene in <i>Nicotiana benthamiana</i> that is related to virus resistance. The transcriptional level of NbCXE expression was significantly increased after <i>Tobacco mosaic virus</i> (TMV) infection. Transient over-expression of NbCXE inhibited TMV  ...[more]

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