Ontology highlight
ABSTRACT:
SUBMITTER: King DM
PROVIDER: S-EPMC7077988 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
King Dana M DM Hong Clarice Kit Yee CKY Shepherdson James L JL Granas David M DM Maricque Brett B BB Cohen Barak A BA
eLife 20200211
In embryonic stem cells (ESCs), a core transcription factor (TF) network establishes the gene expression program necessary for pluripotency. To address how interactions between four key TFs contribute to <i>cis-</i>regulation in mouse ESCs, we assayed two massively parallel reporter assay (MPRA) libraries composed of binding sites for SOX2, POU5F1 (OCT4), KLF4, and ESRRB. Comparisons between synthetic <i>cis</i>-regulatory elements and genomic sequences with comparable binding site configuration ...[more]