Dataset Information

Neutralized Dicalcium Phosphate and Hydroxyapatite Biphasic Bioceramics Promote Bone Regeneration in Critical Peri-Implant Bone Defects.

ABSTRACT: The aim of this study was to evaluate the efficacy of bone regeneration in developed bioceramics composed of dicalcium phosphate and hydroxyapatite (DCP/HA). Critical bony defects were prepared in mandibles of beagles. Defects were grafted using DCP/HA or collagen-enhanced particulate biphasic calcium phosphate (TCP/HA/Col), in addition to a control group without grafting. To assess the efficacy of new bone formation, implant stability quotient (ISQ) values, serial bone labeling, and radiographic and histological percentage of marginal bone coverage (PMBC) were carefully evaluated four, eight, and 12 weeks after surgery. Statistically significant differences among the groups were observed in the histological PMBC after four weeks. The DCP/HA group consistently exhibited significantly higher ISQ values and radiographic and histological PMCB eight and 12 weeks after surgery. At 12 weeks, the histological PMBC of DCP/HA (72.25% ± 2.99%) was higher than that in the TCP/HA/Col (62.61% ± 1.52%) and control groups (30.64% ± 2.57%). After rigorously evaluating the healing of biphasic DCP/HA bioceramics with a critical size peri-implant model with serial bone labeling, we confirmed that neutralized bioceramics exhibiting optimal compression strength and biphasic properties show promising efficacy in fast bone formation and high marginal bone coverage in peri-implant bone defects.

SUBMITTER: Chang HH

PROVIDER: S-EPMC7079663 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Neutralized Dicalcium Phosphate and Hydroxyapatite Biphasic Bioceramics Promote Bone Regeneration in Critical Peri-Implant Bone Defects.

Chang Hao-Hueng HH Yeh Chun-Liang CL Wang Yin-Lin YL Fu Kang-Kuei KK Tsai Shang-Jye SJ Yang Ju-Hsuan JH Lin Chun-Pin CP

Materials (Basel, Switzerland) 20200211 4

The aim of this study was to evaluate the efficacy of bone regeneration in developed bioceramics composed of dicalcium phosphate and hydroxyapatite (DCP/HA). Critical bony defects were prepared in mandibles of beagles. Defects were grafted using DCP/HA or collagen-enhanced particulate biphasic calcium phosphate (TCP/HA/Col), in addition to a control group without grafting. To assess the efficacy of new bone formation, implant stability quotient (ISQ) values, serial bone labeling, and radiographi ...[more]

PMID: 32054126

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Project description:Innovative green, eco-friendly, and biologically derived hydrogels for non-load bearing bone sites were conceived and produced. Natural polysaccharides (copolymers of sodium D-mannuronate and L-guluronate) with natural polypeptides (gelatin) and bioactive mineral fillers (calcium silicates CaSi and dicalcium phosphate dihydrate DCPD) were used to obtain eco-sustainable biomaterials for oral bone defects. Three PP-x:y formulations were prepared (PP-16:16, PP-33:22, and PP-31:31), where PP represents the polysaccharide/polypeptide matrix and x and y represent the weight % of CaSi and DCPD, respectively. Hydrogels were tested for their chemical-physical properties (calcium release and alkalizing activity in deionized water, porosity, solubility, water sorption, radiopacity), surface microchemistry and micromorphology, apatite nucleation in HBSS by ESEM-EDX, FT-Raman, and micro-Raman spectroscopies. The expression of vascular (CD31) and osteogenic (alkaline phosphatase ALP and osteocalcin OCN) markers by mesenchymal stem cells (MSCs) derived from human vascular walls, cultured in direct contact with hydrogels or with 10% of extracts was analysed. All mineral-filled hydrogels, in particular PP-31:31 and PP-33:22, released Calcium ions and alkalized the soaking water for three days. Calcium ion leakage was high at all the endpoints (3 h-28 d), while pH values were high at 3 h-3 d and then significantly decreased after seven days (p < 0.05). Porosity, solubility, and water sorption were higher for PP-31:31 (p < 0.05). The ESEM of fresh samples showed a compact structure with a few pores containing small mineral granules agglomerated in some areas (size 5-20 microns). PP-CTRL degraded after 1-2 weeks in HBSS. EDX spectroscopy revealed constitutional compounds and elements of the hydrogel (C, O, N, and S) and of the mineral powders (Ca, Si and P). After 28 days in HBSS, the mineral-filled hydrogels revealed a more porous structure, partially covered with a thicker mineral layer on PP-31:31. EDX analyses of the mineral coating showed Ca and P, and Raman revealed the presence of B-type carbonated apatite and calcite. MSCs cultured in contact with mineral-filled hydrogels revealed the expression of genes related to vascular (CD31) and osteogenic (mainly OCN) differentiation. Lower gene expression was found when cells were cultured with extracts added to the culture medium. The incorporation of biointeractive mineral powders in a green bio-derived algae-based matrix allowed to produce bioactive porous hydrogels able to release biologically relevant ions and create a suitable micro-environment for stem cells, resulting in interesting materials for bone regeneration and healing in oral bone defects.

Project description:PurposeThe aim of the present systematic review was to evaluate the effect of different lateral bone augmentation (LBA) procedures on the complete correction of a peri-implant bone dehiscence (BD) or fenestration (BF) from implant placement to implant surgical uncovering.MethodsElectronic (Medline, Scopus, and Cochrane databases) and hand literature searches were performed for studies including at least one treatment arm where any LBA had been applied to correct a BD/BF at implant placement (T0). Studies where BD/BF was left untreated were also retrieved as negative control. Data from 24 selected articles were used to perform a network meta-analysis. Based on the proportion of nonresolved BD/BF at implant surgical uncovering (T1), a hierarchy of LBA procedures, and was determined. Spontaneous healing (i.e., exposed implant surface covered by a full-thickness flap; SELF) was also included in the hierarchy. Resorbable membrane + bone graft (RM + BG) was used as reference group. An analysis on the effect of nonhuman (NHBS) vs human (HBS) derived bone substitutes was also performed. NHBS was used as the reference group.ResultsNo statistically significant differences were found among treatments for the proportion of nonresolved BD/BF. SELF performed substantially worse compared to RM + BG (OR: 5.78 × 10, CI: 4.83 × 10 - 1.3 × 1086 ). Treatment based on a combination of a graft material and membrane/periosteum appeared to perform slightly better than treatments using graft material or membrane alone. NHBS appeared to perform better than HBS. SELF had the worst effect among all treatments for both BD/BF height reduction (BDH) and BD/BF width reduction (BDW). Nonresorbable membrane (NRM) and patient's own periosteum (PERI) + BG showed greater increases in buccal bone thickness than RM + BG.ConclusionReconstructive treatment (including use of graft alone, membrane alone, or combinations of grafts and either membrane or patient's own periosteum) of a BD/BF at implant placement favorably and significantly impacts on the probability to obtain complete correction of the BD/BF at implant uncovering when compared to full-thickness flap repositioning on the BD/BF. When using a bone substitute, a nonhuman derived one may be suggested.

Dataset Information

Neutralized Dicalcium Phosphate and Hydroxyapatite Biphasic Bioceramics Promote Bone Regeneration in Critical Peri-Implant Bone Defects.

Publications

Neutralized Dicalcium Phosphate and Hydroxyapatite Biphasic Bioceramics Promote Bone Regeneration in Critical Peri-Implant Bone Defects.

Similar Datasets

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