Dataset Information

The Circadian Clock Gene, TaPRR1, Is Associated With Yield-Related Traits in Wheat (Triticum aestivum L.).

ABSTRACT: Timing of flowering is crucial for the transformation from vegetative to reproductive growth in the important food crop, wheat (Triticum aestivum L.). The circadian clock is a central part of photoperiod regulation, with Pseudo-Response Regulators (PRRs) representing key components of circadian networks. However, little is known about the effects of PRR family members on yield-related traits in crop plants. In this study, we identified polymorphisms and haplotypes of TaPRR1, demonstrating that natural variations in TaPRR1 are associated with significant differences in yield-related traits including heading date, plant height and thousand grain weight. TaPRR1-6A-Hapla showed an earlier heading date, advanced by 0.9 to 1.7%. TaPRR1-6B-Hapla and TaPRR1-6D-Hapla displayed reduced plant height and increased thousand grain weight of up to 13.3 to 26.4% and 6.3 to 17.3%, respectively. Subcellular localization and transcriptional activity analysis showed that TaPRR1 is a nuclear localization protein with transcriptional activity controlled by an IR domain. The expression profiles of TaPRR1 genes over a 48-h period were characterized by circadian rhythms, which had two peaks under both short- and long- day conditions. In addition, geographical distribution analysis indicated higher distribution frequencies of TaPRR1-6A-Hapla, TaPRR1-6B-Haplb, and TaPRR1-6D-Haplb in different agro-ecological production zones. Furthermore, analysis of molecular variance of the distribution frequency of TaPRR1 haplotypes suggested significant differences in haplotype distribution frequency between landraces and modern cultivars. Our study provides a basis for in-depth understanding of TaPRR1 function on yield-related traits in wheat, as well as establishing theoretical guidance for wheat molecular marker-assisted breeding.

SUBMITTER: Sun H

PROVIDER: S-EPMC7080851 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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The Circadian Clock Gene, TaPRR1, Is Associated With Yield-Related Traits in Wheat (Triticum aestivum L.).

Sun Han H Zhang Wenping W Wu Yongzhen Y Gao Lifeng L Cui Fa F Zhao Chunhua C Guo Zhiai Z Jia Jizeng J

Frontiers in plant science 20200312

Timing of flowering is crucial for the transformation from vegetative to reproductive growth in the important food crop, wheat (Triticum aestivum L.). The circadian clock is a central part of photoperiod regulation, with Pseudo-Response Regulators (PRRs) representing key components of circadian networks. However, little is known about the effects of PRR family members on yield-related traits in crop plants. In this study, we identified polymorphisms and haplotypes of TaPRR1, demons ...[more]

PMID: 32226438

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Project description:High vigor seeds have greater yield potential than those with low vigor; however, long-term storage leads to a decline in this trait. The objective of this study was to identify quantitative trait loci (QTLs) for seed vigor-related traits under artificial aging conditions using a high-density genetic linkage map of wheat (Triticum aestivum) and mine the related candidate genes. A doubled haploid population, derived from a cross between Hanxuan 10?×?Lumai 14, was used as the experimental material. Six controlled-environment treatments were set up, i.e. the seeds were aged for 0, 24, 36, 48, 60, and 72 h at a high temperature (48 °C) and under high humidity (relative humidity 100%). Eight traits including seed germination percentage, germination energy, germination index, seedling length, root length, seedling weight, vigor index, and simple vigor index were measured. With the prolongation of artificial aging treatment, these traits showed a continuous downward trend and significant correlations were observed between most of them. A total of 49 additive QTLs for seed vigor-related traits were mapped onto 12 chromosomes (1B, 2D, 3A, 3B, 3D, 4A, 4D, 5A, 5B, 5D, 6D, and 7A); and each one accounted for 6.01-17.18% of the phenotypic variations. Twenty-five pairs of epistatic QTLs were detected on all chromosomes, except for 5D, 6A, and 7D, and each epistasis accounted for 7.35-26.06% of the phenotypic variations. Three additive QTL hot spots were found on chromosomes 5A, 5B, and 5D, respectively. 13 QTLs, QGEe5B, QGIe5B, QSLc5B, QSLd5B, QSLf5B, QRLd5B, QRLe5B, QRLf5B, QVId5B, QVIe5B, QVIf5B, QSVId5B, and QSVIe5B, were located in the marker interval AX-94643729?~?AX-110529646 on 5B and the physical interval 707,412,449-710,959,479 bp. Genes including TRAESCS5B01G564900, TRAESCS5B01G564200, TRAESCS5B01G562600, TraesCS5B02G562700, TRAESCS5B01G561300, TRAESCS5B01G561400, and TRAESCS5B01G562100, located in this marker interval, were found to be involved in regulating the processes of carbohydrate and lipid metabolism, transcription, and cell division during the germination of aging seeds, thus they were viewed as candidate genes for seed viability-related traits. These findings provide the basis for the seed-based cloning and functional identification of related candidate genes for seed vigor.

Dataset Information

The Circadian Clock Gene, TaPRR1, Is Associated With Yield-Related Traits in Wheat (Triticum aestivum L.).

Publications

The Circadian Clock Gene, <i>TaPRR1</i>, Is Associated With Yield-Related Traits in Wheat (<i>Triticum aestivum</i> L.).

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