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Two putative zinc metalloproteases contribute to the virulence of Clostridium perfringens strains that cause avian necrotic enteritis.


ABSTRACT: Two putative zinc metalloproteases encoded by Clostridium perfringens have been implicated in the pathogenesis of necrotic enteritis, an economically significant poultry disease that is caused by this anaerobic bacterium. These proteases have ~64% amino acid identity and are encoded by the zmpA and zmpB genes. We screened 83 C. perfringens isolates by PCR for the presence of these genes. The first gene, zmpB, is chromosomally located and was present in all screened strains of C. perfringens, regardless of their origin and virulence. The second gene, zmpA, is plasmid-borne and was only found in isolates derived from chickens with necrotic enteritis. We describe the generation of insertionally inactivated mutants of both zmpA and zmpB in a virulent C. perfringens isolate. For each mutant, a significant (p?zmpA and zmpB mutants were constructed in different virulent C. perfringens isolates and shown to have reduced virulence in the experimental disease induction model. A zmpA-zmpB double mutant also was generated and shown to have significantly reduced virulence, to the same extent as the respective single mutants. Our results provide evidence that both putative zinc metalloproteases play an important role in disease pathogenesis.

SUBMITTER: Wade B 

PROVIDER: S-EPMC7081491 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Two putative zinc metalloproteases contribute to the virulence of <i>Clostridium perfringens</i> strains that cause avian necrotic enteritis.

Wade Ben B   Keyburn Anthony L AL   Haring Volker V   Ford Mark M   Rood Julian I JI   Moore Robert J RJ  

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 20200110 2


Two putative zinc metalloproteases encoded by <i>Clostridium perfringens</i> have been implicated in the pathogenesis of necrotic enteritis, an economically significant poultry disease that is caused by this anaerobic bacterium. These proteases have ~64% amino acid identity and are encoded by the <i>zmpA</i> and <i>zmpB</i> genes. We screened 83 <i>C. perfringens</i> isolates by PCR for the presence of these genes. The first gene, <i>zmpB</i>, is chromosomally located and was present in all scre  ...[more]

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