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Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes.


ABSTRACT: In Trypanosoma cruzi, the causal agent of Chagas disease, the first seven steps of glycolysis are compartmentalized in glycosomes, which are authentic but specialized peroxisomes. Besides glycolysis, activity of enzymes of other metabolic processes have been reported to be present in glycosomes, such as ?-oxidation of fatty acids, purine salvage, pentose-phosphate pathway, gluconeogenesis and biosynthesis of ether-lipids, isoprenoids, sterols and pyrimidines. In this study, we have purified glycosomes from T. cruzi epimastigotes, collected the soluble and membrane fractions of these organelles, and separated peripheral and integral membrane proteins by Na2CO3 treatment and osmotic shock. Proteomic analysis was performed on each of these fractions, allowing us to confirm the presence of enzymes involved in various metabolic pathways as well as identify new components of this parasite's glycosomes.

SUBMITTER: Acosta H 

PROVIDER: S-EPMC7082770 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes.

Acosta Héctor H   Burchmore Richard R   Naula Christina C   Gualdrón-López Melisa M   Quintero-Troconis Ender E   Cáceres Ana J AJ   Michels Paul A M PAM   Concepción Juan Luis JL   Quiñones Wilfredo W  

Molecular and biochemical parasitology 20190301


In Trypanosoma cruzi, the causal agent of Chagas disease, the first seven steps of glycolysis are compartmentalized in glycosomes, which are authentic but specialized peroxisomes. Besides glycolysis, activity of enzymes of other metabolic processes have been reported to be present in glycosomes, such as β-oxidation of fatty acids, purine salvage, pentose-phosphate pathway, gluconeogenesis and biosynthesis of ether-lipids, isoprenoids, sterols and pyrimidines. In this study, we have purified glyc  ...[more]

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