Unknown

Dataset Information

0

Blue light-triggered photochemistry and cytotoxicity of retinal.


ABSTRACT: The chemical- and photo- toxicity of chromophore retinal on cells have long been debated. Although we recently showed that retinal and blue light exposure interrupt cellular signaling, a comprehensive study examining molecular underpinnings of this perturbation and its consequences to cellular fate is lacking. Here, we report molecular evidence for blue light excited-retinal induced oxidative damage of polyunsaturated lipid anchors in membrane-interacting signaling molecules and DNA damage in cells using live-cell imaging and in vitro experimentation. The incurred molecular damage irreversibly disrupted subcellular localization of these molecules, a crucial criterion for their signaling. We further show retinal accumulation in lipid-bilayers of cell membranes could enhance the lifetime of retinal in cells. Comparative response-signatures suggest that retinal triggers reactions upon photoexcitation similar to photodynamic therapy agents and generate reactive oxygen species in cells. Additionally, data also shows that exposing retinal-containing cells to sunlight induces substantial cytotoxicity. Collectively, our results explain a likely in vivo mechanism and reaction conditions under which bio-available retinal in physiological light conditions damages cells.

SUBMITTER: Ratnayake K 

PROVIDER: S-EPMC7083221 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6033873 | biostudies-literature
| S-EPMC7201882 | biostudies-literature
| S-EPMC7177783 | biostudies-literature
| S-EPMC4258882 | biostudies-literature
| S-EPMC6712202 | biostudies-literature
| S-EPMC2449331 | biostudies-literature
| S-EPMC8389987 | biostudies-literature
| S-EPMC5646837 | biostudies-literature
| S-EPMC6649247 | biostudies-literature
| S-EPMC9047491 | biostudies-literature