Dataset Information

Evidence of a sylvatic enzootic cycle of Leishmania infantum in the State of Amapa, Brazil.

ABSTRACT: INTRODUCTION:Leishmania infantum was considered to be absent from Amapá until 2017 when canine infection was detected. However, there is a lack of knowledge about which reservoir species are involved in transmission in this region. METHODS:Between 2014 and 2016, 86 samples from wild mammals and 74 from domestic dogs were collected in Wajãpi Indigenous Territory and were tested for the presence of deoxyribonucleic acid (DNA) of Leishmania. RESULTS:The DNA of Le. infantum was detected in two rodent samples, Dasyprocta sp. and Proechimys cuvieri. CONCLUSIONS:This is the first evidence characterizing a sylvatic transmission cycle of Le. infantum in the State of Amapá.

SUBMITTER: Moreno ES

PROVIDER: S-EPMC7083388 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Evidence of a sylvatic enzootic cycle of Leishmania infantum in the State of Amapá, Brazil.

Moreno Eduardo Stramandinoli ES Sabioni Luiz Alberto LA Seixas Marina Maria Moraes de MMM Souza Filho Job Alves de JA Marcelino Andreza Pain AP Shimabukuro Paloma Helena Fernandes PHF

Revista da Sociedade Brasileira de Medicina Tropical 20191220

<h4>Introduction</h4>Leishmania infantum was considered to be absent from Amapá until 2017 when canine infection was detected. However, there is a lack of knowledge about which reservoir species are involved in transmission in this region.<h4>Methods</h4>Between 2014 and 2016, 86 samples from wild mammals and 74 from domestic dogs were collected in Wajãpi Indigenous Territory and were tested for the presence of deoxyribonucleic acid (DNA) of Leishmania.<h4>Results</h4>The DNA of Le. infantum was ...[more]

PMID: 31859944

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Project description:BackgroundThe sand fly Nyssomyia neivai is one of the most abundant species in Southern Brazil. It is frequently found in areas that are foci of visceral leishmaniasis in the state of Santa Catarina, caused by Leishmania infantum. In this region, the main vector of L. infantum, Lutzomyia longipalpis, has not been detected. In the absence of L. longipalpis, this study aimed to identify the sand fly fauna and diagnose any potential Leishmania spp. infection in sand flies and in dogs in a region of Southern Brazil that experienced a recent canine visceral leishmaniasis outbreak.MethodsThis report includes a survey of the sand fly fauna at the Zoonosis Control Center of the Municipality of Tubarão (Santa Catarina, Brazil). Molecular tests were conducted to investigate Leishmania spp. natural infection in sand flies using polymerase chain reaction (PCR). In positive females, in addition to morphological identification, molecular analysis through DNA barcoding was performed to determine the sand fly species. Additionally, the dogs were tested for the presence of Leishmania spp. using a non-invasive technique for the collection of biological material, to be assessed by PCR.ResultsA total of 3419 sand flies, belonging to five genera, were collected. Nyssomyia neivai was the most abundant species (85.8%), followed by Migonemyia migonei (13.3%), Pintomyia fischeri (0.8%), Evandromyia edwardsi (< 0.1%), and species of the genus Brumptomyia. (0.1%). Out of the 509 non-engorged females analyzed by PCR, two (0.4%) carried L. infantum DNA. The naturally infected females were identified as Ny. neivai, in both morphological and molecular analysis. In addition, two out of 47 conjunctival swabs from dogs tested positive for L. infantum, yielding an infection rate of 4.2%.ConclusionsThese results confirm the presence of Ny. neivai naturally infected with L. infantum in an area where dogs were also infected by the parasite, suggesting its potential role as a vector in Southern Brazil.

Dataset Information

Evidence of a sylvatic enzootic cycle of Leishmania infantum in the State of Amapa, Brazil.

Publications

Evidence of a sylvatic enzootic cycle of Leishmania infantum in the State of Amapá, Brazil.

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