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Epigenetic Regulation of WNT3A Enhancer during Regeneration of Injured Cortical Neurons.

ABSTRACT: Traumatic brain injury is known to reprogram the epigenome. Chromatin immunoprecipitation-sequencing of histone H3 lysine 27 acetylation (H3K27ac) and tri-methylation of histone H3 at lysine 4 (H3K4me3) marks was performed to address the transcriptional regulation of candidate regeneration-associated genes. In this study, we identify a novel enhancer region for induced WNT3A transcription during regeneration of injured cortical neurons. We further demonstrated an increased mono-methylation of histone H3 at lysine 4 (H3K4me1) modification at this enhancer concomitant with a topological interaction between sub-regions of this enhancer and with promoter of WNT3A gene. Together, this study reports a novel mechanism for WNT3A gene transcription and reveals a potential therapeutic intervention for neuronal regeneration.

SUBMITTER: Chang CY

PROVIDER: S-EPMC7084788 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Epigenetic Regulation of <i>WNT3A</i> Enhancer during Regeneration of Injured Cortical Neurons.

Chang Chu-Yuan CY Hung Jui-Hung JH Huang Liang-Wei LW Li Joye J Fung Ka Shing KS Kao Cheng-Fu CF Chen Linyi L

International journal of molecular sciences 20200310 5

Traumatic brain injury is known to reprogram the epigenome. Chromatin immunoprecipitation-sequencing of histone H3 lysine 27 acetylation (H3K27ac) and tri-methylation of histone H3 at lysine 4 (H3K4me3) marks was performed to address the transcriptional regulation of candidate regeneration-associated genes. In this study, we identify a novel enhancer region for induced <i>WNT3A</i> transcription during regeneration of injured cortical neurons. We further demonstrated an increased mono-methylatio ...[more]

PMID: 32164275

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