Improved detection of esp, hyl, asa1, gelE, cylA virulence genes among clinical isolates of Enterococci.
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ABSTRACT:
SUBMITTER: Kiruthiga A
PROVIDER: S-EPMC7085142 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Publications
Improved detection of esp, hyl, asa1, gelE, cylA virulence genes among clinical isolates of Enterococci.
Kiruthiga Alexander A Padmavathy Kesavaram K Shabana Praveen P Naveenkumar Venkatesan V Gnanadesikan Sumathi S Malaiyan Jeevan J
BMC research notes 20200320 1
<h4>Objective</h4>Virulence factors (VFs) among the clinical strains of enterococci play a vital role in pathogenesis. This study was aimed to screen for cylA, asa1, gelE, esp and hyl among Enterococcus faecalis (n = 89) and E. faecium (n = 51) by multiplex PCR. The previously reported multiplex PCR was modified to 2 duplex (asa1 and gelE, cylA and esp) PCRs and 1 simplex (hyl) PCR. The idea of the modification of the multiplex PCR proposed here emerged in the course of the research study when m ...[more]
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