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Generation of seven induced pluripotent stem cell lines from neonates of different ethnic backgrounds.

ABSTRACT: Seven human induced pluripotent stem cell (iPSC) lines were generated from fibroblasts from three neonatal individuals using non-integrative reprogramming. Most control iPSCs are derived from adults, so these iPSCs meet the need for control iPSCs from young individuals. Donors were from different ethnicities and these lines provide unique genetic profiles. All iPSCs have normal karyotypes, express stem cell markers, and exhibit pluripotency, as assessed by capacity to differentiate into three germ layers. These lines are valuable to study human development, as age-matched controls for disorder-specific iPSCs, and as platforms for gene editing to control for age and ethnicity.

SUBMITTER: Yin Y

PROVIDER: S-EPMC7086470 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Generation of seven induced pluripotent stem cell lines from neonates of different ethnic backgrounds.

Yin Yingnan Y Petersen Andrew J AJ Soref Cheryl C Richards William D WD Ludwig Tenneille T Taapken Seth S Berndt Erich E Zhang Su-Chun SC Bhattacharyya Anita A

Stem cell research 20181220

Seven human induced pluripotent stem cell (iPSC) lines were generated from fibroblasts from three neonatal individuals using non-integrative reprogramming. Most control iPSCs are derived from adults, so these iPSCs meet the need for control iPSCs from young individuals. Donors were from different ethnicities and these lines provide unique genetic profiles. All iPSCs have normal karyotypes, express stem cell markers, and exhibit pluripotency, as assessed by capacity to differentiate into three ge ...[more]

PMID: 30605841

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