Dataset Information

Characterization of avian influenza viruses isolated from domestic ducks in Vietnam in 2009 and 2010.

ABSTRACT: In the surveillance of avian influenza in Vietnam, 26 H9N2, 1 H3N2, 1 H3N8, 7 H4N6, 3 H11N3, and 1 H11N9 viruses were isolated from tracheal and cloacal swab samples of 300 domestic ducks in April 2009, and 1 H9N6 virus from 300 bird samples in March 2010. Out of the 27 H9 virus isolates, the hemagglutinins of 18 strains were genetically classified as belonging to the sublineage G1, and the other nine belonged to the Korean sublineage. Phylogenetic analysis revealed that one of the 27 H9 viruses was a reassortant in which the PB2 gene belonged to the Korean sublineage and the other seven genes belonged to the G1 sublineage. Three representative H9N2 viruses were intranasally inoculated into ducks, chickens, pigs, and mice. On the basis of experimental infection studies, it was found that each of the three viruses readily infected pigs and replicated in their upper respiratory tracts, and they infected chickens with slight replication. Viruses were recovered from the lungs of mice inoculated with two of the three isolates. The present results reveal that H9 avian influenza viruses are prevailing and genetic reassortment occurs among domestic ducks in Vietnam. It is recommended that careful surveillance of swine influenza with H9 viruses should be performed to prepare for pandemic influenza.

SUBMITTER: Nomura N

PROVIDER: S-EPMC7086777 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Characterization of avian influenza viruses isolated from domestic ducks in Vietnam in 2009 and 2010.

Nomura Naoki N Sakoda Yoshihiro Y Endo Mayumi M Yoshida Hiromi H Yamamoto Naoki N Okamatsu Masatoshi M Sakurai Kenji K Hoang Nam Van NV Nguyen Long Van LV Chu Huy Duc HD Tien Tien Ngoc TN Kida Hiroshi H

Archives of virology 20111109 2

In the surveillance of avian influenza in Vietnam, 26 H9N2, 1 H3N2, 1 H3N8, 7 H4N6, 3 H11N3, and 1 H11N9 viruses were isolated from tracheal and cloacal swab samples of 300 domestic ducks in April 2009, and 1 H9N6 virus from 300 bird samples in March 2010. Out of the 27 H9 virus isolates, the hemagglutinins of 18 strains were genetically classified as belonging to the sublineage G1, and the other nine belonged to the Korean sublineage. Phylogenetic analysis revealed that one of the 27 H9 viruses ...[more]

PMID: 22068881

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Project description:Ducks are a natural reservoir of influenza A viruses (IAVs) and can act as a reassortment vessel. Wetlands, such as Hakaluki and Tanguar haor in Bangladesh, have unique ecosystems including domestic duck (Anas platyrhynchos domesticus) rearing, especially household and free-range ducks. A cross-sectional study was, therefore, conducted to explore avian influenza status and its distribution and risk factors in the wetland areas. During the three consecutive winters of 2015-2017, specifically in December of these years, we collected a total of 947 samples including blood, oropharyngeal and cloacal swabs from domestic ducks (free-range duck (n = 312 samples) and household ducks (n = 635 samples) in wetlands. We screened serum samples using a nucleoprotein competitive enzyme-linked immunosorbent assay (c-ELISA) to estimate seroprevalence of IAV antibodies and swab samples by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) to detect IA viral M gene. Eleven (11) M gene positive samples were subjected to sequencing and phylogenetic analysis. Serological and viral prevalence rates of IAVs were 63.8% (95% CI: 60.6-66.8) and 10.7% (8.8-12.8), respectively. Serological and viral RNA prevalence rates were 51.8% (95% CI: 47.2-56.4) and 10.2% (7.6-13.3) in Hakaluki haor, 75.6% (71.5-79.4) and 11.1% (8.5-14.3) in Tanguar haor, 66.3% (62.5-69.9) and 11.2% (8.8-13.9) in household ducks and 58.7% (52.9-64.2) and 9.6% (6.5-13.4) in free-range ducks, respectively. The risk factors identified for higher odds of AI seropositive ducks were location (OR = 2.9, 95% CI: 2.2-3.8, p < 0.001; Tanguar haor vs. Hakaluki haor), duck-rearing system (OR = 1.4, 1.1-1.8, household vs. free-range), farmer's education status (OR = 1.5, 1.2-2.0, p < 0.05 illiterate vs. literate) and contact type (OR = 3.0, 2.1-4.3, p < 0.001; contact with chicken vs. no contact with chicken). The risk factors identified for higher odds of AI RNA positive ducks were farmer's education status (OR = 1.5, 1.0-2.3, p < 0.05 for illiterate vs literate), contact type (OR = 2.7, 1.7-4.2, p < 0.001; ducks having contact with chicken vs. ducks having contact with waterfowl). The phylogenetic analysis of 11 partial M gene sequences suggested that the M gene sequences detected in free-range duck were very similar to each other and were closely related to the M gene sequences of previously reported highly pathogenic avian influenza (HPAI) and low pathogenic avian influenza (LPAI) subtypes in waterfowl in Bangladesh and Southeast Asian countries. Results of the current study will help provide significant information for future surveillance programs and model IAV infection to predict the spread of the viruses among migratory waterfowl, free-range ducks and domestic poultry in Bangladesh.

Dataset Information

Characterization of avian influenza viruses isolated from domestic ducks in Vietnam in 2009 and 2010.

Publications

Characterization of avian influenza viruses isolated from domestic ducks in Vietnam in 2009 and 2010.

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