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Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China.


ABSTRACT: Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006-2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups.

SUBMITTER: Chen J 

PROVIDER: S-EPMC7087116 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China.

Chen Jianfei J   Liu Xiaozhen X   Lang Hongwu H   Wang Zhongtian Z   Shi Da D   Shi Hongyan H   Zhang Xin X   Feng Li L  

Archives of virology 20130205 6


Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006-2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95  ...[more]

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