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A novel strategy for analyzing RNA-protein interactions by surface plasmon resonance biosensor.


ABSTRACT: Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in this paper. In our study, it has been shown that the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) has a high binding affinity for the leader sequence of SARS-CoV genome. Effect of temperature on the RNA-DNA hybridization was also examined. This method can provide the affinity of interactions with high sensitivity. Therefore, it will be useful in screening binding candidates for a given RNA target motif with one chip.

SUBMITTER: Yang Y 

PROVIDER: S-EPMC7090661 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

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A novel strategy for analyzing RNA-protein interactions by surface plasmon resonance biosensor.

Yang Yaling Y   Wang Quan Q   Guo Deyin D  

Molecular biotechnology 20080509 1


Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in this paper. In our study, it has been shown that the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) has a high binding affinity for the leader se  ...[more]

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