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Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT.

ABSTRACT: Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.

SUBMITTER: Fielding BC 

PROVIDER: S-EPMC7092935 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature

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Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT.

Fielding Burtram C BC   Gunalan Vithiagaran V   Tan Timothy H P TH   Chou Chih-Fong CF   Shen Shuo S   Khan Sehaam S   Lim Seng Gee SG   Hong Wanjin W   Tan Yee-Joo YJ  

Biochemical and biophysical research communications 20060324 4

Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and resu  ...[more]

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