Ontology highlight
ABSTRACT:
SUBMITTER: Hsieh MS
PROVIDER: S-EPMC7094261 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Journal of immunological methods 20180609
A bi-cistronic baculovirus expression vector was constructed to facilitate the expression, detection, and isolation of the hemagglutinin (HA) fragment HA1 of H6N1 avian influenza virus (AIV) in an insect and a culture of its cells. In this construct, the GP67sp signal peptide promoted the secretion of the recombinant protein into the culture medium, and improved protein expression and purification. Enhanced green fluorescent protein, co-expressed through an internal ribosome entry site, served a ...[more]