Ontology highlight
ABSTRACT:
SUBMITTER: Lindner HA
PROVIDER: S-EPMC7094341 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Lindner Holger A HA Lytvyn Viktoria V Qi Hongtao H Lachance Paule P Ziomek Edmund E Ménard Robert R
Archives of biochemistry and biophysics 20070714 1
The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV PLpro) carries out N-terminal processing of the viral replicase polyprotein, and also exhibits Lys48-linked polyubiquitin chain debranching and ISG15 precursor processing activities in vitro. Here, we used SDS-PAGE and fluorescence-based assays to demonstrate that ISG15 derivatives are the preferred substrates for the deubiquitinating activity of the PLpro. With k(cat)/K(M) of 602,000 M(-1)s(-1), PLpro hydrolyzes IS ...[more]