Unknown

Dataset Information

0

Overexpression of pulmonary extracellular superoxide dismutase attenuates endotoxin-induced acute lung injury.


ABSTRACT:

Purpose

Superoxide is produced by activated neutrophils during the inflammatory response to stimuli such as endotoxin, can directly or indirectly injure host cells, and has been implicated in the pathogenesis of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). We wished to determine the potential for pulmonary overexpression of the extracellular isoform of superoxide dismutase (EC-SOD) to reduce the severity of endotoxin-induced lung injury.

Methods

Animals were randomly allocated to undergo intratracheal instillation of (1) surfactant alone (vehicle); (2) adeno-associated virus (AAV) vectors containing a null transgene (AAV-null); and (3) adeno-associated virus vectors containing the EC-SOD transgene (AAV-EC-SOD) and endotoxin was subsequently administered intratracheally. Two additional groups were randomized to receive (1) vehicle or (2) AAV-EC-SOD, and to undergo sham (vehicle) injury. The severity of the lung injury was assessed in all animals 24 h later.

Results

Endotoxin produced a severe lung injury compared to sham injury. The AAV vector encoding EC-SOD increased lung EC-SOD concentrations, and enhanced the antioxidant capacity of the lung. EC-SOD overexpression decreased the severity of endotoxin-induced ALI, reducing the decrement in systemic oxygenation and lung compliance, decreasing lung permeability and decreasing histologic injury. EC-SOD attenuated pulmonary inflammation, decreased bronchoalveolar lavage neutrophil counts, and reduced interleukin-6 and CINC-1 concentrations. The AAV vector itself did not contribute to inflammation or to lung injury.

Conclusions

Pulmonary overexpression of EC-SOD protects the lung against endotoxin-induced ALI.

SUBMITTER: Hassett P 

PROVIDER: S-EPMC7095197 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Overexpression of pulmonary extracellular superoxide dismutase attenuates endotoxin-induced acute lung injury.

Hassett Patrick P   Curley Gerard F GF   Contreras Maya M   Masterson Claire C   Higgins Brendan D BD   O'Brien Timothy T   Devaney James J   O'Toole Daniel D   Laffey John G JG  

Intensive care medicine 20110714 10


<h4>Purpose</h4>Superoxide is produced by activated neutrophils during the inflammatory response to stimuli such as endotoxin, can directly or indirectly injure host cells, and has been implicated in the pathogenesis of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). We wished to determine the potential for pulmonary overexpression of the extracellular isoform of superoxide dismutase (EC-SOD) to reduce the severity of endotoxin-induced lung injury.<h4>Methods</h4>Animals were  ...[more]

Similar Datasets

| S-EPMC2633057 | biostudies-literature
| S-EPMC3202580 | biostudies-literature
| S-EPMC2290736 | biostudies-literature
2020-05-29 | GSE147138 | GEO
| S-EPMC7705200 | biostudies-literature
| S-EPMC2685504 | biostudies-literature
| S-EPMC3888358 | biostudies-literature
| S-EPMC1153619 | biostudies-other