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Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes.


ABSTRACT: Stem cell-derived extracellular vesicles (EVs) offer alternative approaches to stem cell-based therapy for regenerative medicine. In this study, stem cell EVs derived during differentiation are developed to use as cell-free therapeutic systems by inducing tissue-specific differentiation. EVs are isolated from human adipose-derived stem cells (HASCs) during white and beige adipogenic differentiation (D-EV and BD-EV, respectively) via tangential flow filtration. D-EV and BD-EV can successfully differentiate HASCs into white and beige adipocytes, respectively. D-EV are transplanted with collagen/methylcellulose hydrogels on the backs of BALB/c mice, and they produce numerous lipid droplets in injected sites. Treatments of BD-EV attenuate diet-induced obesity through browning of adipose tissue in mice. Furthermore, high-fat diet-induced hepatic steatosis and glucose tolerance are improved by BD-EV treatment. miRNAs are responsible for the observed effects of BD-EV. These results reveal that secreted EVs during stem cell differentiation into white adipocytes or beige adipocytes can promote cell reprogramming.

SUBMITTER: Jung YJ 

PROVIDER: S-EPMC7096171 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes.

Jung Youn Jae YJ   Kim Hark Kyun HK   Cho Yoonsuk Y   Choi Ji Suk JS   Woo Chang Hee CH   Lee Kyoung Soo KS   Sul Jae Hoon JH   Lee Chan Mi CM   Han Jihoon J   Park Jae Hyung JH   Jo Dong-Gyu DG   Cho Yong Woo YW  

Science advances 20200325 13


Stem cell-derived extracellular vesicles (EVs) offer alternative approaches to stem cell-based therapy for regenerative medicine. In this study, stem cell EVs derived during differentiation are developed to use as cell-free therapeutic systems by inducing tissue-specific differentiation. EVs are isolated from human adipose-derived stem cells (HASCs) during white and beige adipogenic differentiation (D-EV and BD-EV, respectively) via tangential flow filtration. D-EV and BD-EV can successfully dif  ...[more]

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