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Renal expression and urinary excretion of liver-type fatty acid-binding protein in cats with renal disease.

ABSTRACT: BACKGROUND:Liver-type fatty acid-binding protein (L-FABP) is a biomarker for early detection of renal disease in humans. Liver-type fatty acid-binding protein is cytotoxic oxidation products secreted from proximal tubules under ischemia and oxidative stress. OBJECTIVE:To examine renal expression and quantify urinary excretion of L-FABP in catswith renal disease. ANIMALS:One hundred and thirty-four client-owned cats including 34 cats with serum creatinine (sCre) values >1.6?mg/dL and 10 other cats that died in clinics. METHODS:Tissue expressions of L-FABP were examined by reverse transcription polymerase chain reaction and Western blotting. Urinary L-FABP (uL-FABP) and serum L-FABP (sL-FABP) levels were determined by enzyme-linked immunosorbent assay. Anti-liver-type fatty acid-binding protein antibody immunostained renal sections. RESULTS:Feline kidneys express L-FABP. Strong L-FABP signals were observed in the lumens of proximal tubular cells in 5 cats with high uL-FABP excretion, but not in 5 cats with low uL-FABP excretion. In 9 normal cats, uL-FABP index was <1.2??g/g urinary creatinine (uCre). High uL-FABP indexes (>10.0??g/g uCre) were detected in 7 of 100 cats with low sCre (<1.6?mg/dL) and 18 of 44 cats with high sCre (>1.6?mg/dL). There was a weak correlation between L-FABP index and sCre, serum symmetric dimethylarginine (SDMA), or blood urea nitrogen (BUN), and these correlation coefficients were increased by analyzing only data of cats with sCre >1.6?mg/dL. There was a weak correlation between u L-FABP index and sL-FABP in all tested cats, but not in cats with high sCre. CONCLUSIONS AND CLINICAL IMPORTANCE:This study demonstrates correlations between L-FABP and current renal biomarkers for chronic kidney disease in cats, such as sCre and SDMA. Liver-type fatty acid-binding protein may be a potential biomarker to predict early pathophysiological events in feline kidneys.

SUBMITTER: Katayama M

PROVIDER: S-EPMC7096645 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Renal expression and urinary excretion of liver-type fatty acid-binding protein in cats with renal disease.

Katayama Masaaki M Ohata Keiichi K Miyazaki Tamako T Katayama Rieko R Wakamatsu Nobuko N Ohno Misa M Yamashita Tetsuro T Oikawa Tsuyoshi T Sugaya Takeshi T Miyazaki Masao M

Journal of veterinary internal medicine 20200222 2

<h4>Background</h4>Liver-type fatty acid-binding protein (L-FABP) is a biomarker for early detection of renal disease in humans. Liver-type fatty acid-binding protein is cytotoxic oxidation products secreted from proximal tubules under ischemia and oxidative stress.<h4>Objective</h4>To examine renal expression and quantify urinary excretion of L-FABP in catswith renal disease.<h4>Animals</h4>One hundred and thirty-four client-owned cats including 34 cats with serum creatinine (sCre) values >1.6 ...[more]

PMID: 32087614

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Project description:BACKGROUND: Renal cell carcinoma (RCC) is the most common renal neoplasm. Cancer tissue is often characterized by altered energy regulation. Fatty acid-binding proteins (FABP) are involved in the intracellular transport of fatty acids (FA). We examined the level of brain-type (B) and liver-type (L) FABP mRNA and the protein expression profiles of both FABPs in renal cell carcinoma. METHODS: Paired tissue samples of cancerous and noncancerous kidney parts were investigated. Quantitative RT-PCR, immunohistochemistry and western blotting were used to determine B- and L-FABP in tumor and normal tissues. The tissue microarray (TMA) contained 272 clinico-pathologically characterized renal cell carcinomas of the clear cell, papillary and chromophobe subtype. SPSS 17.0 was used to apply crosstables (chi2-test), correlations and survival analyses. RESULTS: B-FABP mRNA was significantly up-regulated in renal cell carcinoma. In normal tissue B-FABP mRNA was very low or often not detectable. RCC with a high tumor grading (G3 + G4) showed significantly lower B-FABP mRNA compared with those with a low grading (G1 + G2). Western blotting analysis detected B-FABP in 78% of the cases with a very strong band but in the corresponding normal tissue it was weak or not detectable. L-FABP showed an inverse relationship for mRNA quantification and western blotting. A strong B-FABP staining was present in 52% of the tumor tissues contained in the TMA. In normal renal tissue, L-FABP showed a moderate to strong immunoreactivity in proximal tubuli. L-FABP was expressed at lower rates compared with the normal tissues in 30.5% of all tumors. There was no correlation between patient survival times and the staining intensity of both FABPs. CONCLUSION: While B-FABP is over expressed in renal cell carcinoma in comparison to normal renal tissues L-FABP appears to be reduced in tumor tissue. Although the expression behavior was not related to the survival outcome of the RCC patients, it can be assumed that these changes indicate fundamental alterations in the fatty metabolism in the RCC carcinogenesis. Further studies should identify the role of both FABPs in carcinogenesis, progression and with regard to a potential target in RCC.

Dataset Information

Renal expression and urinary excretion of liver-type fatty acid-binding protein in cats with renal disease.

Publications

Renal expression and urinary excretion of liver-type fatty acid-binding protein in cats with renal disease.

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