Agreement of stall-side and laboratory major crossmatch tests with the reference standard method in horses.

ABSTRACT:

Background

Crossmatching is used to prevent life-threatening transfusion reactions in horses. Laboratory methods are laborious and technically challenging, which is impractical during emergencies.

Hypothesis/objectives

Evaluate agreement between a stall-side crossmatch kit (KIT) and a laboratory method (LAB) in horses with known and unknown blood types.

Animals

Twenty-four blood-typed and alloantibody-screened healthy adult horses (Aim 1) and 156 adult horses of unknown blood type (Aim 2).

Methods

Prospective, blinded study. Expected positive (n = 35) and negative (n = 36) crossmatches among 24 antibody and blood-typed horses were used to determine sensitivity and specificity of KIT and LAB against the reference method. Agreement in 156 untyped horses was evalu

SUBMITTER: Fenn MS 

PROVIDER: S-EPMC7096648 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature