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Harnessing iron-oxide nanoparticles towards the improved bactericidal activity of macrophage against Staphylococcus aureus.

ABSTRACT: Iron oxide nanoparticles (IONPs) have been increasingly used in various biomedical applications in preclinical and clinical settings. Although the interactions of IONPs with macrophages have been well-reported in the context of nanoparticle toxicity, harnessing the capacity of IONPs in reprograming macrophages towards bactericidal activity has not been explored. Here, using an in vitro culture model of macrophages and an in vivo mouse model of skin wound infection by Staphylococcus aureus (S. aureus), we demonstrated that IONPs in combination with a strategy to trigger the Fenton reaction could significantly enhance bactericidal effects of macrophages against intracellular S. aureus by inducing a M1 macrophage polarization that stimulates the production of reactive oxygen species. Our study supports that harnessing the characteristic of IONPs to tune macrophage polarization to exhibit a bactericidal activity may provide a new strategy for treating infectious diseases.

SUBMITTER: Yu B

PROVIDER: S-EPMC7098381 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Harnessing iron-oxide nanoparticles towards the improved bactericidal activity of macrophage against Staphylococcus aureus.

Yu Bing B Wang Zhongxia Z Almutairi Layla L Huang Songping S Kim Min-Ho MH

Nanomedicine : nanotechnology, biology, and medicine 20200123

Iron oxide nanoparticles (IONPs) have been increasingly used in various biomedical applications in preclinical and clinical settings. Although the interactions of IONPs with macrophages have been well-reported in the context of nanoparticle toxicity, harnessing the capacity of IONPs in reprograming macrophages towards bactericidal activity has not been explored. Here, using an in vitro culture model of macrophages and an in vivo mouse model of skin wound infection by Staphylococcus aureus (S. au ...[more]

PMID: 31982615

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Project description:This study was designed to determine the effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the biological activity of a bacterial biofilm (Streptococcus mutans). Our hypothesis was that the diffusion of the SPIONs into biofilms would depend on their surface properties, which in turn would largely be determined by their surface functionality. Bare, positively charged and negatively charged SPIONs, with hydrodynamic diameters of 14.6 ± 1.4 nm, 20.4 ± 1.3 nm and 21.2 ± 1.6 nm were evaluated. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) and electrophoretic mobility (EPM) measurements were used to confirm that carboxylic functional groups predominated on the negatively charged SPIONS, whereas amine functional groups predominated on the positively charged particles. Transmission electron microscopy (TEM) showed the morphology and sizes of SPIONs. Scanning electron microscopy (SEM) and EPM measurements indicated that the surfaces of the SPIONs were covered with biomolecules following their incubation with the biofilm. Bare SPIONs killed bacteria less than the positively charged SPIONs at the highest exposure concentrations, but the toxicity of the bare and positively charged SPIONs was the same for lower SPION concentrations. The positively charged SPIONs were more effective in killing bacteria than the negatively charged ones. Nonetheless, electrophoretic mobilities of all three SPIONs (negative, bare and positively charged) became more negative following incubation with the (negatively-charged) biofilm. Therefore, while the surface charge of SPIONS was important in determining their biological activity, the initial surface charge was not constant in the presence of the biofilm, leading eventually to SPIONS with fairly similar surface charges in situ. The study nonetheless suggests that the surface characteristics of the SPIONS is an important parameter controlling the efficiency of antimicrobial agents. The analysis of the CFU/mL values shows that the SPIONs have the same toxicity on bacteria in solution in comparison with that on the biofilm.

Dataset Information

Harnessing iron-oxide nanoparticles towards the improved bactericidal activity of macrophage against Staphylococcus aureus.

Publications

Harnessing iron-oxide nanoparticles towards the improved bactericidal activity of macrophage against Staphylococcus aureus.

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