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Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30.


ABSTRACT: Enhancing cellulase production in Trichoderma reesei is of great interest for an economical biorefinery. Artificial transcription factors are a potentially powerful molecular strategy for improving cellulase production in T. reesei. In this study, enhanced transcriptional activators XYR1VP, ACE2VP, and ACE1VP were constructed by linking the C terminus of XYR1, ACE2, or ACE1 with an activation domain of herpes simplex virus protein VP16. T. reesei transformants TXYR1VP, TACE2VP, and TACE1VP showed improved cellulase and/or xylanase production. TXYR1VP has a cellulase-free phenotype but with significantly elevated xylanase production. Xylanase I and xylanase II activities [U/(mg biomass)] increased by 51% and 80%, respectively, in TXYR1VP in comparison with parental strain RUT C30. The filter paper activity of TACE2VP in the Avicel-based medium increased by 52% compared to that of RUT C30. In the Avicel-based medium, TACE1VP manifested an 80% increase in FPase activity and a 50% increase in xylanase activity as compared to those of RUT C30. Additionally, when pretreated corn stover was hydrolyzed, crude enzymes produced from TACE1VP yielded a greater glucose release than did the enzymes produced by parental strain RUT C30.

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7101855 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Construction of enhanced transcriptional activators for improving cellulase production in <i>Trichoderma reesei</i> RUT C30.

Zhang Jiajia J   Wu Chuan C   Wang Wei W   Wang Wei W   Wei Dongzhi D  

Bioresources and bioprocessing 20180818 1


Enhancing cellulase production in <i>Trichoderma reesei</i> is of great interest for an economical biorefinery. Artificial transcription factors are a potentially powerful molecular strategy for improving cellulase production in <i>T. reesei</i>. In this study, enhanced transcriptional activators XYR1VP, ACE2VP, and ACE1VP were constructed by linking the C terminus of XYR1, ACE2, or ACE1 with an activation domain of herpes simplex virus protein VP16. <i>T. reesei</i> transformants T<sub>XYR1VP</  ...[more]

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