Ontology highlight
ABSTRACT:
SUBMITTER: Ahn J
PROVIDER: S-EPMC7103880 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Molecules and cells 20200301 3
Cryo-electron microscopy (cryo-EM) is now the first choice to determine the high-resolution structures of huge protein complexes. Grids with two-dimensional arrays of holes covered with a carbon film are typically used in cryo-EM. Although semi-automatic plungers are available, notable trial-and-error is still required to obtain a suitable grid specimen. Herein, we introduce a new method to obtain thin ice specimens using real-time measurement of the liquid amounts in cryo-EM grids. The grids fo ...[more]