Improved Isolation of Uncultured Anaerobic Bacteria using Medium Prepared with Separate Sterilization of Agar and Phosphate.
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ABSTRACT:
SUBMITTER: Kato S
PROVIDER: S-EPMC7104283 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
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Improved Isolation of Uncultured Anaerobic Bacteria using Medium Prepared with Separate Sterilization of Agar and Phosphate.
Kato Souichiro S Terashima Mia M Yama Ayano A Sato Megumi M Kitagawa Wataru W Kawasaki Kosei K Kamagata Yoichi Y
Microbes and environments 20200101 1
We previously demonstrated that a simple modification in the preparation of agar media, i.e., autoclaving phosphate and agar separately (termed the "PS protocol"), improved the culturability of aerobic microorganisms by reducing the generation of reactive oxygen species. We herein investigated the effects of the PS protocol on the cultivation of anaerobic microorganisms using sludge from a wastewater treatment system as a microbial source. The application of the PS protocol increased colony numb ...[more]
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