Dataset Information

Acoustic Cell Separation Based On Biophysical Properties.

ABSTRACT: Density and mechanical properties (e.g., compressibility or bulk modulus) are important cellular biophysical markers. As a result, developing a method to separate cells directly based on these properties benefits various applications including biological research, diagnosis, prognosis, and medical therapy. As a potential solution, surface acoustic wave (SAW) based cell separation has demonstrated advantages in terms of biocompatibility and compact device size. However, most SAW-reliant cell separations are achieved using an entangled effect of density, various mechanical properties, and size. In this work, we demonstrate SAW-based separation of cells/particles based on their densities and mechanical properties, irrespective of their sizes. Manipulating the acoustic properties of the fluidic medium allows us to decouple the effects of size from other mechanical properties during the separation process. Using our platform, SAW based separation is achieved by varying the dimensions of the microfluidic channels, the wavelengths of acoustic signals, and the properties of the fluid media in the microfluidic domain. Our method was applied to separate paraformaldehyde-treated and fresh Hela cells based on differences in mechanical properties; a recovery rate of 85% for fixed cells was achieved. It was also applied to separate red blood cells (RBCs) and white blood cells (WBCs) which have different densities. A recovery rate of 80.5% for WBCs was achieved. Owing to its excellent biocompatibility and simple design, our acoustic-based separation method can provide a valuable tool for fundamental research, diagnostics, drug efficacy assessments, and therapeutics.

SUBMITTER: Xie Y

PROVIDER: S-EPMC7104781 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Publications

Acoustic Cell Separation Based on Density and Mechanical Properties.

Xie Yuliang Y Mao Zhangming Z Bachman Hunter H Li Peng P Zhang Peiran P Ren Liqiang L Wu Mengxi M Huang Tony Jun TJ

Journal of biomechanical engineering 20200301 3

Density and mechanical properties (e.g., compressibility or bulk modulus) are important cellular biophysical markers. As such, developing a method to separate cells directly based on these properties can benefit various applications including biological research, diagnosis, prognosis, and therapeutics. As a potential solution, surface acoustic wave (SAW)-based cell separation has demonstrated advantages in terms of biocompatibility and compact device size. However, most SAW-reliant cell separati ...[more]

PMID: 32006021

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Project description:In droplet-based microfluidic platforms, precise separation of microscale droplets of different chemical composition is increasingly necessary for high-throughput combinatorial chemistry in drug discovery and screening assays. A variety of droplet sorting methods have been proposed, in which droplets of the same kind are translocated. However, there has been relatively less effort in developing techniques to separate the uniform-sized droplets of different chemical composition. Most of the previous droplet sorting or separation techniques either rely on the droplet size for the separation marker or adopt on-demand application of a force field for the droplet sorting or separation. The existing droplet microfluidic separation techniques based on the in-droplet chemical composition are still in infancy because of the technical difficulties. In this study, we propose an acoustofluidic method to simultaneously separate microscale droplets of the same volume and dissimilar acoustic impedance using ultrasonic surface acoustic wave (SAW)-induced acoustic radiation force (ARF). For extensive investigation on the SAW-induced ARF acting on both cylindrical and spherical droplets, we first performed a set of the droplet sorting experiments under varying conditions of acoustic impedance of the dispersed phase fluid, droplet velocity, and wave amplitude. Moreover, for elucidation of the underlying physics, a new dimensionless number ARD was introduced, which was defined as the ratio of the ARF to the drag force acting on the droplets. The experimental results were comparatively analyzed by using a ray acoustics approach and found to be in good agreement with the theoretical estimation. Based on the findings, we successfully demonstrated the simultaneous separation of uniform-sized droplets of the different acoustic impedance under continuous application of the acoustic field in a label-free and detection-free manner. Insomuch as on-chip, precise separation of multiple kinds of droplets is critical in many droplet microfluidic applications, the proposed acoustofluidic approach will provide new prospects for microscale droplet separation.

Dataset Information

Acoustic Cell Separation Based On Biophysical Properties.

Publications

Acoustic Cell Separation Based on Density and Mechanical Properties.

Similar Datasets

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