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Genetic diversity of human rhinoviruses in Cambodia during a three-year period reveals novel genetic types.

ABSTRACT: Acute respiratory viral infections are a major cause of morbidity during early childhood in developing countries. Human rhinoviruses are the most frequent cause of upper respiratory tract infections in humans, which can range in severity from asymptomatic to clinically severe disease. In this study we collected 4170 nasopharyngeal swabs from patients hospitalised with influenza-like illness in two Cambodian provincial hospitals between 2007 and 2010. Samples were screened for 18 respiratory viruses using 5 multiplex PCRs. A total of 11.2% of samples tested positive for human rhinoviruses (HRV). VP4/2 and VP1 regions were amplified and sequenced to study the distribution of rhinoviruses genotypes and species in Cambodia during this three-year period. Five novel genotypes, 2 species A, 2 species B and 1 species C were identified based on VP1 sequences. Co-infections with other viruses were demonstrated.

SUBMITTER: Naughtin M

PROVIDER: S-EPMC7105975 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Genetic diversity of human rhinoviruses in Cambodia during a three-year period reveals novel genetic types.

Naughtin Monica M Sareth Rith R Sentilhes Anne-Charlotte AC Vong Sirenda S Joffret Marie-Line ML Cornillot Emmanuel E Deubel Vincent V Delpeyroux Francis F Frutos Roger R Buchy Philippe P

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 20150729

Acute respiratory viral infections are a major cause of morbidity during early childhood in developing countries. Human rhinoviruses are the most frequent cause of upper respiratory tract infections in humans, which can range in severity from asymptomatic to clinically severe disease. In this study we collected 4170 nasopharyngeal swabs from patients hospitalised with influenza-like illness in two Cambodian provincial hospitals between 2007 and 2010. Samples were screened for 18 respiratory viru ...[more]

PMID: 26231720

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Genetic diversity of human rhinoviruses in Cambodia during a three-year period reveals novel genetic types.

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Genetic diversity of human rhinoviruses in Cambodia during a three-year period reveals novel genetic types.

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