Dataset Information

Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.

ABSTRACT: BACKGROUND:Currently, RT-PCR is used widely and considered to be a convenient, useful, and powerful method for molecular diagnosis, to detect pathogens from clinical specimens. OBJECTIVES:In this work we describe the development of an in-house Real-time Taqman PCR assay for quantification of HPeV in stool specimens. STUDY DESIGNS:A total of 137 fecal specimens previously screened for rotavirus and adenovirus were tested for HPeV virus. RESULTS:A total of 11 out of 137 (8%) episodes of acute gastroenteritis were associated with HPeV genomic detection with median viral load 14678±28927 genomes/mg fecal specimens. There was no significant difference in the detection rate between male and female (54.5% (6/11) vs. 45.5% (5/11). Among the 11 HPeV-positive cases, 2 were also positive for other viral pathogens, including rotavirus (n=2). CONCLUSION:In conclusion, the development of a laboratory designed Real Time PCR TaqMan assay for quantitative detection of HPeV and the optimization and standardization of this assay using stool of children with acute gastroenteritis are described.

SUBMITTER: Bergallo M

PROVIDER: S-EPMC7106367 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.

Bergallo Massimiliano M Galliano Ilaria I Montanari Paola P Rosa Brusin Martina M Gabiano Clara C

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 20161026

<h4>Background</h4>Currently, RT-PCR is used widely and considered to be a convenient, useful, and powerful method for molecular diagnosis, to detect pathogens from clinical specimens.<h4>Objectives</h4>In this work we describe the development of an in-house Real-time Taqman PCR assay for quantification of HPeV in stool specimens.<h4>Study designs</h4>A total of 137 fecal specimens previously screened for rotavirus and adenovirus were tested for HPeV virus.<h4>Results</h4>A total of 11 out of 13 ...[more]

PMID: 27816019

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Project description:IntroductionDiarrheal diseases constitute a significant public health problem in terms of mortality and morbidity. In Honduras and around the world, RVs have consistently emerged as the single most important etiologic agent in acute childhood diarrhea. However, other viruses, such as NoVs and HAstVs, have also been shown to be responsible for viral gastroenteritis. Unfortunately, the country has limited information concerning the etiologic role of these viral agents in acute gastroenteritis. This study investigated the frequency, genotypes, and epidemiological characteristics of RV-A, NoVs, and HAstVs among children under 5 years old in Distrito Central, Honduras.MethodsStool samples and their corresponding epidemiological data were collected from children with acute gastroenteritis in three healthcare centers in Distrito Central. All samples were screened by immunoassays for RV-A and HAstVs. RV-A-positive samples were molecularly characterized by RT-PCR and genotyping assays. RT-PCR was also applied to confirm HAstVs positivity and to detect NoVs, followed by nucleotide sequencing to assign their genotypes.ResultsOur results show that at least one viral agent was detected in 31% of the children. The frequency of RV-A, NoVs, and HAstVs was 14%, 13%, and 5%, respectively. The most frequent RV-A genotype was G2P[4], occurring in 93% of cases. 92.3% of NoVs-positive samples belonged to genogroup II, with GII.4 and GII.16 being the most common. HAstVs were clustered into three genotypes: HAstV-1, HAstV-2, and HAstV-8. Only one sample showed coinfection with NoVs and HAstVs.ConclusionThis comprehensive molecular and epidemiological characterization of enteric viruses demonstrates the vast diversity of these agents and describes for the first time NoVs and HAstVs as causative agents of acute childhood gastroenteritis in Distrito Central, Honduras. This suggests that further in-depth studies of the pediatric population are necessary to develop and implement effective preventive and control measures in the country.

Dataset Information

Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.

Publications

Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.

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