Dataset Information

Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.

ABSTRACT: BACKGROUND:Currently, RT-PCR is used widely and considered to be a convenient, useful, and powerful method for molecular diagnosis, to detect pathogens from clinical specimens. OBJECTIVES:In this work we describe the development of an in-house Real-time Taqman PCR assay for quantification of HPeV in stool specimens. STUDY DESIGNS:A total of 137 fecal specimens previously screened for rotavirus and adenovirus were tested for HPeV virus. RESULTS:A total of 11 out of 137 (8%) episodes of acute gastroenteritis were associated with HPeV genomic detection with median viral load 14678±28927 genomes/mg fecal specimens. There was no significant difference in the detection rate between male and female (54.5% (6/11) vs. 45.5% (5/11). Among the 11 HPeV-positive cases, 2 were also positive for other viral pathogens, including rotavirus (n=2). CONCLUSION:In conclusion, the development of a laboratory designed Real Time PCR TaqMan assay for quantitative detection of HPeV and the optimization and standardization of this assay using stool of children with acute gastroenteritis are described.

SUBMITTER: Bergallo M

PROVIDER: S-EPMC7106367 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.

Bergallo Massimiliano M Galliano Ilaria I Montanari Paola P Rosa Brusin Martina M Gabiano Clara C

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 20161026

<h4>Background</h4>Currently, RT-PCR is used widely and considered to be a convenient, useful, and powerful method for molecular diagnosis, to detect pathogens from clinical specimens.<h4>Objectives</h4>In this work we describe the development of an in-house Real-time Taqman PCR assay for quantification of HPeV in stool specimens.<h4>Study designs</h4>A total of 137 fecal specimens previously screened for rotavirus and adenovirus were tested for HPeV virus.<h4>Results</h4>A total of 11 out of 13 ...[more]

PMID: 27816019

Similar Datasets

Project description:BackgroundDiarrhea in children under five years of age remains a challenge in reducing child mortality in Nepal. Understanding the spatiotemporal patterns and influencing factors of the disease is important for control and intervention.MethodsData regarding diarrhea prevalence and its potential influencing factors were extracted from the Demographic and Health Surveys in Nepal and other open-access databases. A Bayesian logistic regression model with district-specific spatio-temporal random effects was applied to explore the space and time patterns of diarrhea risk, as well as the relationships between the risk and the potential influencing factors.ResultsBoth the observed prevalence and the estimated spatiotemporal effects show a decreasing diarrhea risk trend from 2006 to 2016 in most districts of Nepal, with a few exceptions, such as Achham and Rasuwa. The disease risk decreased with mothers' years of education (OR 0.93, 95% Bayesian Credible Interval (BCI) 0.87, 0.997). Compared to spring, autumn and winter had lower risks of diarrhea. The risk firstly increased and then decreased with age and children under 12-24 months old were the highest risk group (OR 1.20, 95% BCI 1.04, 1.38). Boys had higher risk than girls (OR 1.24, 95% BCI 1.13, 1.39). Even though improved sanitation wasn't found significant within a 95% BCI, there was 93.2% of chance of it being a protective factor. There were no obvious spatiotemporal clusters among districts and each district tended to have its own spatiotemporal diarrhea prevalence pattern.ConclusionsThe important risk factors identified by our Bayesian spatial-temporal modeling provide insights for control and intervention on children diarrhea in Nepal. Special attention should be paid to high risk groups of children and high risk seasons, as well as districts with high risk or increased trend of risk. Effective actions should be implemented to improve sanitation and women's education level. District-specific control planning is recommended for local governments for effective control of children diarrhea in Nepal.

Dataset Information

Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.

Publications

Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure