Dataset Information

Molecular dissection of Secale africanum chromosome 6R^afr in wheat enabled localization of genes for resistance to powdery mildew and stripe rust.

ABSTRACT:

Background

Introgression of chromatin from Secale species into common wheat has for decades been a successful strategy for controlling the wheat diseases. The wild Secale species, Secale africanum Stapf., is a valuable source for resistance to foliar disease of wheat. A wheat-S. africanum chromosome 6R^afr substitution line displayed resistance to both powdery mildew and stripe rust at the adult-plant stage.

Results

Wheat-S. africanum chromosome 6R^afr deletion and translocation lines were produced and identified by sequential non-denaturing fluorescence in situ hybridization (ND-FISH) using multiple Oligo-based probes. Different ND-FISH patterns were observed between S. cereale 6R and S. africanum 6R^afr. With reference to the physical map of the draft genome sequence of rye inbred line Lo7, a comprehensive PCR marker analysis indicated that insertions and deletions had occurred by random exchange between chromosomes 6R and 6R^afr. A survey of the wheat- S. africanum 6R^afr lines for disease resistance indicated that a powdery mildew resistance gene(s) was present on the long arm of 6R^afr at FL0.85-1.00, and that a stripe rust resistance gene(s) was located in the terminal region of 6R^afrS at FL0.95-1.00. The wheat-S. africanum 6R^afr introgression lines also displayed superior agronomic traits, indicating that the chromosome 6R^afr may have little linkage drag in the wheat background.

Conclusions

The combination of molecular and cytogenetic methods allowed to precisely identify the chromosome rearrangements in wheat- S. africanum 6R^afr substitution, deletion and translocation lines, and compare the structural difference between chromosomes 6R and 6R^afr. The wheat- S. africanum 6R^afr lines containing gene(s) for powdery mildew and stripe rust resistance could be used as novel germplasm for wheat breeding by chromosome engineering.

SUBMITTER: Li G

PROVIDER: S-EPMC7106737 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Molecular dissection of Secale africanum chromosome 6R<sup>afr</sup> in wheat enabled localization of genes for resistance to powdery mildew and stripe rust.

Li Guangrong G Tang Lingrong L Yin Yan Y Zhang Ahui A Yu Zhihui Z Yang Ennian E Tang Zongxiang Z Fu Shulan S Yang Zujun Z

BMC plant biology 20200331 1

<h4>Background</h4>Introgression of chromatin from Secale species into common wheat has for decades been a successful strategy for controlling the wheat diseases. The wild Secale species, Secale africanum Stapf., is a valuable source for resistance to foliar disease of wheat. A wheat-S. africanum chromosome 6R<sup>afr</sup> substitution line displayed resistance to both powdery mildew and stripe rust at the adult-plant stage.<h4>Results</h4>Wheat-S. africanum chromosome 6R<sup>afr</sup> deletion ...[more]

PMID: 32234016

Similar Datasets

Project description:BackgroundStripe rust (Puccinia striiformis f. sp. tritici; Pst) and powdery mildew (Blumeria graminis f. sp. tritici; Bgt) are important diseases of wheat (Triticum aestivum) worldwide. Similar mechanisms and gene transcripts are assumed to be involved in the host defense response because both pathogens are biotrophic fungi. The main objective of our study was to identify co-regulated mRNAs that show a change in expression pattern after inoculation with Pst or Bgt, and to identify mRNAs specific to the fungal stress response.ResultsThe transcriptome of the hexaploid wheat line N9134 inoculated with the Chinese Pst race CYR 31 was compared with that of the same line inoculated with Bgt race E09 at 1, 2, and 3 days post-inoculation. Infection by Pst and Bgt affected transcription of 23.8% of all T. aestivum genes. Infection by Bgt triggered a more robust alteration in gene expression in N9134 compared with the response to Pst infection. An array of overlapping gene clusters with distinctive expression patterns provided insight into the regulatory differences in the responses to Bgt and Pst infection. The differentially expressed genes were grouped into seven enriched Kyoto Encyclopedia of Genes and Genomes pathways in Bgt-infected leaves and four pathways in Pst-infected leaves, while only two pathways overlapped. In the plant-pathogen interaction pathway, N9134 activated a higher number of genes and pathways in response to Bgt infection than in response to Pst invasion. Genomic analysis revealed that the wheat genome shared some microbial genetic fragments, which were specifically induced in response to Bgt and Pst infection.ConclusionsTaken together, our findings indicate that the responses of wheat N9134 to infection by Bgt and Pst shows differences in the pathways and genes activated. The mass sequence data for wheat-fungus interaction generated in this study provides a powerful platform for future functional and molecular research on wheat-fungus interactions.