Ontology highlight
ABSTRACT:
SUBMITTER: Lansing F
PROVIDER: S-EPMC7107906 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Lansing Felix F Paszkowski-Rogacz Maciej M Schmitt Lukas Theo LT Schneider Paul Martin PM Rojo Romanos Teresa T Sonntag Jan J Buchholz Frank F
Nucleic acids research 20200101 1
Site-specific recombinases (SSRs) such as the Cre/loxP system are useful genome engineering tools that can be repurposed by altering their DNA-binding specificity. However, SSRs that delete a natural sequence from the human genome have not been reported thus far. Here, we describe the generation of an SSR system that precisely excises a 1.4 kb fragment from the human genome. Through a streamlined process of substrate-linked directed evolution we generated two separate recombinases that, when exp ...[more]