Antigenicity analysis of different regions of the severe acute respiratory syndrome coronavirus nucleocapsid protein.

ABSTRACT:

Background

The widespread threat of severe acute respiratory syndrome (SARS) to human health has made urgent the development of fast and accurate analytical methods for its early diagnosis and a safe and efficient antiviral vaccine for preventive use. For this purpose, we investigated the antigenicity of different regions of the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein.

Methods

The cDNA for full-length N protein and its various regions from the SARS-CoV was cloned and expressed in Escherichia coli. After purification, all of the protein fragments were printed on glass slides to fabricate a protein microarray and then probed with the sera from SARS patients to determine the reactivity of these protein fragments.

Results

The full-length protein and two other

SUBMITTER: Chen Z 

PROVIDER: S-EPMC7108132 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature