Ontology highlight
ABSTRACT:
SUBMITTER: Zhang XW
PROVIDER: S-EPMC7108759 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Zhang Xin-Wei XW Hatamie Amir A Ewing Andrew G AG
Journal of the American Chemical Society 20200224 9
We have developed the means to simultaneously measure the physical size and count catecholamine molecules in individual nanometer transmitter vesicles. This is done by combining resistive pulse (RP) measurements in a nanopore pipet and vesicle impact electrochemical cytometry (VIEC) at an electrode as the vesicle exits the nanopore. Analysis of freshly isolated bovine adrenal vesicles shows that the size and internal catecholamine concentration of vesicles varies with the occurrence of a dense c ...[more]