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A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function.

ABSTRACT: Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-terminal mitochondrial localization sequence derived from the D. discoideum protein GcvH1 (glycine cleavage system H1) attached to a red fluorescent protein enables clear mitochondrial imaging. We also show that this labelling has no effect upon mitochondria load or respiratory function.

SUBMITTER: Perry CJ

PROVIDER: S-EPMC7109396 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function.

Perry Christopher J CJ Warren Eleanor C EC Damstra-Oddy Joseph L JL Storey Claire C Francione Lisa M LM Annesley Sarah J SJ Fisher Paul R PR Müller-Taubenberger Annette A Williams Robin S B RSB

Biochemistry and biophysics reports 20200325

Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-terminal mitochondrial localization sequence derived from the D. discoideum protein GcvH1 (glycine cleavage system H1) attached to a red fluorescent protein enables clear mitochondrial ima ...[more]

PMID: 32258439

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